[IF=4.2] Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).  IHC-P ;  Mouse.  

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[IF=3.73] Majchrzak K, Lo Re D, Gajewska M, Bulkowska M, Homa A, et al. (2013) Migrastatin Analogues Inhibit Canine Mammary Cacer Cell Migrationand Invasion. PLoS ONE 8(10): e76789  Dog.  

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[IF=3.31] Król, Magdalena, et al. "Macrophages Mediate a Switch between Canonical and Non-Canonical Wnt Pathways in Canine Mammary Tumors." PloS one 9.1 (2014): e83995.  Dog.  

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[IF=0] Haokang Zhang. et al. The Actin Crosslinker Fascin Regulates Cell Chirality. Advanced Biology. 2023 Jan;:2200240  WB ;  Mouse.  

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Sample: U87MG(Human) Cell Lysate at 30 ug Primary: Anti-phospho-FSCN1(Ser39) (bs-0772R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD

Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Testis tissue lysates Lane 3: Mouse Spleen tissue lysates Lane 4: Rat Cerebrum tissue lysates Lane 5: Rat Testis tissue lysates Lane 6: Rat Spleen tissue lysates Lane 7: Human HepG2 cell lysates Lane 8: Human K562 cell lysates Lane 9: Human SH-SY5Y cell lysates Lane 10: Human U-2os cell lysates Primary: Anti- phospho-FSCN1 (Ser39) (bs-0772R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 51 kDa

Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FSCN1(Ser39)) Polyclonal Antibody, Unconjugated (bs-0772R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: musle of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-phospho-FSCN1(Ser39) Polyclonal Antibody, Unconjugated(bs-0772R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-FSCN1 (Ser39)) polyclonal Antibody, Unconjugated (bs-0772R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Blank control: U937(blue) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 3μl in 100 μL1X PBS containing 0.5% BSA(green).