| 产品编号 | bs-0689R |
| 英文名称 | Rabbit Anti-PEPT1 antibody |
| 中文名称 | 肠道肽转运蛋白1/小肽转运蛋白1抗体 |
| 别 名 | SLC15A1; Oligopeptide transporter, small intestine isoform; Peptide transporter 1; Intestinal H(+)/peptide cotransporter; Solute carrier family 15 member 1(SLC15A1); +peptide cotransporter; HPECT1; HPEPT1; Human peptide transporter(HPEPT1) mRNA complete cds; Intestinal H; Oligopeptide transporter small intestine isoform; PEPT1; Peptide transporter 1; Solute carrier family 15(oligopeptide transporter) member 1; S15A1_HUMAN. |
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Specific References (5) | bs-0689R has been referenced in 5 publications.
[IF=10.317] Yaxian Zheng. et al. Tailored elasticity combined with biomimetic surface promotes nanoparticle transcytosis to overcome mucosal epithelial barrier. Biomaterials. 2020 Dec;262:120323 IF ; Human.
[IF=7.514] Yi Wu. et al. Selective transportation and energy homeostasis regulation of dietary advanced glycation end-products in human intestinal Caco-2 cells. FOOD CHEM. 2022 Oct;391:133284 FC ; Human.
[IF=6.383] Yang Wen. et al. Different copper sources and levels affect growth performance, copper content, carcass characteristics, intestinal microorganism and metabolism of finishing pigs. Anim Nutr. 2021 Nov;: WB ; Pig.
[IF=3.73] Xu, Qingbiao, et al. "Establishment and Characterization of an Omasal Epithelial Cell Model Derived from Dairy Calves for the Study of Small Peptide Absorption." PLOS ONE 9.3 (2014): e88993. Bovine.
[IF=3.357] Morita M et al. Fluorescence‐based discrimination of breast cancer cells by direct exposure to 5‐aminolevulinic acid. Cancer Med. 2019 Aug 5. WB ; Human.
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| 研究领域 | 信号转导 通道蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-1000,IHC-P=1:50-200,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 79 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PEPT1: 101-200/714(chk) <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
PEPT1(Oligopeptide transporter, small intestine isoform (Peptide transporter 1) (Intestinal H(+)/peptide cotransporter) (Solute carrier family 15 member 1) Proton-coupled intake of oligopeptides of 2 to 4 amino acids with a preference for dipeptides. May constitute a major route for the absorption of protein digestion end-products. Subcellular Location：membrane; Multi-pass membrane protein. Tissue Specificity Intestine, kidney, liver and low in brain. Belongs to the PTR2/POT transporter (TC 2.A.17) family. Function: Proton-coupled intake of oligopeptides of 2 to 4 amino acids with a preference for dipeptides. May constitute a major route for the absorption of protein digestion end-products. Subcellular Location: Membrane; Multi-pass membrane protein. Similarity: Belongs to the PTR2/POT transporter (TC 2.A.17) family. SWISS: P46059 Gene ID: 6564 Database links: Entrez Gene: 6564 Human Omim: 600544 Human SwissProt: P46059 Human Unigene: 436893 Human 小肽转运蛋白又称寡肽转运蛋白; 小肽是蛋白质在动物体内消化的主要产物，而位于小肠刷状缘膜上的小肽转运蛋白 在小肽的吸收过程中发挥重要作用，因此PepT1的活性直接影响小肽的吸收。 PepT1主要在消化道中表达，在肾脏中也有微弱的表达。PepT1是低親和力、高容量的肽載體PepT2是高親和力、低容量的肽載體。PepT2主要在腎臟中表達。 |
| 产品图片 |
Sample:
Lane 1: Liver (Mouse) Lysate at 40 ug
Lane 2: Liver (Rat) Lysate at 40 ug
Lane 3: Panc-1 (Human) Cell Lysate at 30 ug
Primary: Anti-SLC15A1 (bs-0689R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 83 kD
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PEPT1 Polyclonal Antibody, Unconjugated (bs-0689R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PEPT1 Polyclonal Antibody, Unconjugated (bs-0689R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PEPT1 Polyclonal Antibody, Unconjugated (bs-0689R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PEPT1 Polyclonal Antibody, Unconjugated (bs-0689R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PEPT1 Polyclonal Antibody, Unconjugated (bs-0689R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PEPT1 Polyclonal Antibody, Unconjugated (bs-0689R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
