| 产品编号 | bs-0185R |
| 英文名称 | Rabbit Anti-PDGF-B antibody |
| 中文名称 | 血小板源性生长因子-B抗体 |
| 别 名 | PDGF beta; PDGF-B; Platelet-derived growth factor B-chain; PDGF AB + BB; Becaplermin; c sis; FLJ1285; Oncogene SIS; PDGF 1; PDGF 2; PDGF A; PDGF A chain; PDGF B chain; PDGF subunit A; PDGF subunit B; PDGF1; PDGF2; PDGFA; PDGFB; PDGFR1; Platelet derived growth factor 2; Platelet derived growth factor alpha; Platelet derived growth factor alpha chain; Platelet derived growth factor alpha polypeptide; Platelet derived growth factor B chain; Platelet derived growth factor beta; Platelet derived growth factor beta polypeptide; Platelet derived growth factor subunit A; Platelet derived growth factor subunit B; SIS; SSV; v sis platelet derived growth factor beta polypeptide. |
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Specific References (8) | bs-0185R has been referenced in 8 publications.
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| 研究领域 | 心血管 信号转导 生长因子和激素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Sheep,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 27kDa |
| 细胞定位 | 细胞核 细胞浆 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PDGF-B: 155-210/241 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heal the wound. [SUBUNIT] Antiparallel disulfide-linked dimer of nonidentical (A and B) chains. Homodimers of A and B chains are implicated in transformation processes. A-A and B-B, as well as A-B, dimers can bind to the PDGF receptor. Belongs to the PDGF/VEGF growth factor family. Function: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. Subunit: Homodimer; antiparallel disulfide-linked dimer. Heterodimer with PDGFA; antiparallel disulfide-linked dimer. The PDGFB homodimer interacts with PDGFRA and PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with XLKD1. Subcellular Location: Secreted. Note=Released by platelets upon wounding. Tissue Specificity: Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung. DISEASE: Note=A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB. Similarity: Belongs to the PDGF/VEGF growth factor family. SWISS: P01127 Gene ID: 5155 Database links: Entrez Gene: 5155 Human Entrez Gene: 18591 Mouse Omim: 190040 Human SwissProt: P01127 Human SwissProt: P31240 Mouse Unigene: 1976 Human Unigene: 144089 Mouse Unigene: 198230 Rat 生长因子和激素（ Growth Factor and Hormones） PDGF（platelet-dirived growth factor）是一种碱性蛋白，分为两个亚基（A、B链）通过二硫键结合而成的两条多肽链的异二聚体，分子量为28-35kDa。为促细胞分裂剂，可使小鼠成纤维细胞及其他多种细胞增殖。最新研究发现了PDGF家族两种新的基因PDGF-C、PDGF-D。 |
| 产品图片 |
Sample:
Stomach (Rat) Lysate at 40 ug
Primary: Anti-PDGF-B (bs-0185R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 32 kD
Sample:
Lane 1: Kidney (Mouse) Lysate at 40 ug
Lane 2: Cerebrum (Mouse) Lysate at 40 ug
Lane 3: Cerebrum (Rat) Lysate at 40 ug
Lane 4: Lung (Mouse) Lysate at 40 ug
Lane 5: Lung (Rat) Lysate at 40 ug
Lane 6: Huvec (Human) Cell Lysate at 30 ug
Lane 7: A431 (Human) Cell Lysate at 30 ug
Lane 8: MCF-7 (Human) Cell Lysate at 30 ug
Lane 9: U2os (Human) Cell Lysate at 30 ug
Primary: Anti-PDGF-B (bs-0185R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 28 kD
Tissue/cell: rat transplant lymphoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PDGF-B Polyclonal Antibody, Unconjugated(bs-0071R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (blue line):Hela(blue).
Primary Antibody (green line): Rabbit Anti-PDGF-B antibody (bs-0185R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
