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Rabbit Anti-P-cadherin  antibody (bs-1159R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-1159R
英文名称 P-cadherin
中文名称 P-钙粘附分子抗体
别    名 placental; Cadherin-3; P cadherin; Cadp; Placental cadherin; P-cadherin; AI385538; Pca; Cadherin 3 precursor; Cadherin 3 type 1; Calcium dependent adhesion protein placental; Cadherin; CDH3; CDHP; 7B4 antigen; Cadherin-1; Cadherin-2; Cadherin-4; Cadherin-5; CAM 120/80; CD144; CD324; CD325; Cdh4; CDH5; CDHE; CDHN; CADH3_HUMAN; E-cadherin; Epithelial cadherin; N-cadherin; NCAD; Neural cadherin; P-cadherin; UVO; Uvomorulin; Vascular endothelial cadherin; CDH 3; CDH3; CDH3 protein; CDHP; HJMD; PCAD; Placental cadherin.   
Specific References  (1)     |     bs-1159R has been referenced in 1 publications.
[IF=5.232] Chen F et al. Integrated Analysis of Quantitative Proteome and Transcriptional Profiles Reveals the Dynamic Function of Maternally Expressed Proteins After Parthenogenetic Activation of Buffalo Oocyte.Mol Cell Proteomics. 2018 Oct;17(10):1875-1891.  WB ;  Buffalo.  
研究领域 细胞生物  免疫学  细胞粘附分子  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human (predicted: Mouse,Rat,Chicken,Dog,Cow,Rabbit,GuineaPig)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, Flow-Cyt=2μg/Test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 80kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human P-cadherin: 625-725/829 <Cytoplasmic>
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍
cell-cell adhesion molecules. Cadherins are responsible for a whole range of processes including development, wound healing, cell-cell signaling, cell growth and differentiation. N-cadherin is found in many locations including cardiac adherins junctions, oral squamous epithelial cells, and breast epithelial cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Function:
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Subunit:
Interacts with CDCP1.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein.

Tissue Specificity:
Expressed in some normal epithelial tissues and in some carcinoma cell lines.

DISEASE:
Defects in CDH3 are the cause of hypotrichosis with juvenile macular dystrophy (HJMD) [MIM:601553]. HJMD is a rare autosomal recessive disorder characterized by early hair loss heralding severe degenerative changes of the retinal macula and culminating in blindness during the second to third decade of life.
Defects in CDH3 are the cause of ectodermal dysplasia with ectrodactyly and macular dystrophy (EEM) [MIM:225280]; also known as EEM syndrome, Albrectsen-Svendsen syndrome or Ohdo-Hirayama-Terawaki syndrome. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EEM is an autosomal recessive condition characterized by features of ectodermal dysplasia such as sparse eyebrows and scalp hair, and selective tooth agenesis associated with macular dystrophy and ectrodactyly.

Similarity:
Contains 5 cadherin domains.

SWISS:
P22223

Gene ID:
1001

Database links:

Entrez Gene: 1001 Human

Omim: 114021 Human

SwissProt: P22223 Human

Unigene: 191842 Human



产品图片
Sample:
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti-P-cadherin (bs-1159R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 90 kD
Tissue/cell: U251 cells;4% Paraformaldehyde-fixed;
Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-P-cadherin Polyclonal Antibody, Unconjugated(bs-1159R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
Image provided by Independent Validation (badge 029741). Histogram of human DU145 and human LNCaP cells stained with Rabbit Anti-P-cadherin Polyclonal Antibody (orange)(bs-1159R at 1:100), isotype control antibody (green), secondary antibody only (blue) and unstained (red)
Overlay histogram showing Mouse SP2/0 stained with bs-1159R-PE (red line). The cells were fixed with 1% paraformaldehyde (10 min). The cells were then incubated with the antibody(bs-1159R-PE, 2ug/1x106cells) for 30 min at 22-25°C. Isotype control antibody (black line) was rabbit IgG (2ug/1x106cells) used under the same conditions. Acquisition of >5,000 events was performed.
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