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Rabbit Anti-Thrombin receptor/ PAR1 antibody (bs-0828R)
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说明书: 50ul  100ul  200ul
50ul/780.00元
100ul/1380.00元
200ul/2200.00元
大包装/询价

产品编号 bs-0828R
英文名称 Thrombin receptor/ PAR1
中文名称 蛋白酶激活受体-1抗体
别    名 SAR; BXR; Proteinase-activated receptor 1 precursor; Thrombin receptor; Coagulation factor II receptor; D15S227E; Prader-Willi/Angelman region-1; PAR1; Coagulation factor II receptor; CF2R; Coagulation factor II (thrombin) receptor; F2R; HTR; PAR 1; PAR1; Protease activated receptor 1; Proteinase activated receptor 1; Thrombin receptor; TR; PAR-1; Coagulation factor II receptor; Coagulation factor II receptor; PAR-1; PAR1_HUMAN; Proteinase activated receptor 1; Proteinase-activated receptor 1.  
Specific References  (3)     |     bs-0828R has been referenced in 3 publications.
[IF=3.01] Liu, Rui, et al. "Identification of FLOT2 as a novel target for microRNA-34a in melanoma." Journal of Cancer Research and Clinical Oncology (2014): 1-14.  WB ;  Human.  
[IF=2.31] Fan, Hai-Xia, et al. "Expression of MMP-1/PAR-1 and patterns of invasion in oral squamous cell carcinoma as potential prognostic markers." OncoTargets and therapy 8 (2015): 1619.  IHC-P ;  Human.  
[IF=3.53] Acton, David, and Gareth B. Miles. "Stimulation of Glia Reveals Modulation of Mammalian Spinal Motor Networks by Adenosine." PLOS ONE 10.8 (2015): e0134488.  IHC-P ;  Mouse.  
规格价格 50ul/780元 购买    100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 50ul  100ul  200ul
研究领域 肿瘤  细胞生物  信号转导  激酶和磷酸酶  细胞膜受体  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 42kDa
细胞定位 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PAR1:251-350/425 <Cytoplasmic>
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. [provided by RefSeq, Jul 2008]

Function:
High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Platelets and vascular endothelial cells.

Post-translational modifications:
A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
Phosphorylated; probably mediating desensitization prior to the uncoupling and internalization of the receptor.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
P25116

Gene ID:
2149

Database links:

Entrez Gene: 2149 Human

Omim: 187930 Human

SwissProt: P56488 Baboon

SwissProt: P25116 Human

Unigene: 482562 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PAR蛋白全称为蛋白酶体活化受体(Protease-activated receptors),又称:凝血酶受体(Thrombin Receptor)是独立家族的G蛋白偶联受体, 研究认为与炎症,实体肿瘤等密切相关。
PAR1蛋白是乳腺癌细胞侵润和迁移的必需因子,它是作为基质金属蛋白酶体1(MMP-1)的受体关键性作用,这一研究结果为研制新的抗癌药物提供新的靶点。
产品图片
Sample:
K562(Human) Cell Lysate at 30 ug
Primary: Anti-PAR1 (bs-0828R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
Brain (Rat) Lysate at 30 ug
Liver (Mouse) Lysate at 30 ug
Primary: Anti- PAR-1 (bs-0828R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 42kD
Observed band size : 44kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thrombin receptor/ PAR1) Polyclonal Antibody, Unconjugated (bs-0828R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thrombin receptor PAR1) Polyclonal Antibody, Unconjugated (bs-0828R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Tissue/cell: human mammary cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PAR1 Polyclonal Antibody, Unconjugated(bs-0828R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PAR1 Polyclonal Antibody, Unconjugated(bs-0828R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PAR-1 Polyclonal Antibody, Unconjugated(bs-0828R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat colitis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PAR-1 Polyclonal Antibody, Unconjugated(bs-0828R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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