| 产品编号 | bs-0828R |
| 英文名称 | Rabbit Anti-PAR1 antibody |
| 中文名称 | 蛋白酶激活受体-1抗体 |
| 别 名 | SAR; PAR-1; PAR 1; BXR; CF2R; F2R; HTR; TR; Proteinase-activated receptor 1 precursor; Proteinase activated receptor 1; Thrombin receptor; Coagulation factor II receptor; Prader-Willi/Angelman region-1; Coagulation factor II (thrombin) receptor; PAR1_HUMAN; |
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Specific References (12) | bs-0828R has been referenced in 12 publications.
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| 研究领域 | 肿瘤 细胞生物 信号转导 激酶和磷酸酶 细胞膜受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 42kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PAR1: 251-350/425 <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. [provided by RefSeq, Jul 2008] Function: High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Platelets and vascular endothelial cells. Post-translational modifications: A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand. Phosphorylated; probably mediating desensitization prior to the uncoupling and internalization of the receptor. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P25116 Gene ID: 2149 Database links: Entrez Gene: 2149 Human Omim: 187930 Human SwissProt: P56488 Baboon SwissProt: P25116 Human Unigene: 482562 Human PAR蛋白全称为蛋白酶体活化受体(Protease-activated receptors)，又称：凝血酶受体(Thrombin Receptor)是独立家族的G蛋白偶联受体， 研究认为与炎症，实体肿瘤等密切相关。 PAR1蛋白是乳腺癌细胞侵润和迁移的必需因子，它是作为基质金属蛋白酶体1（MMP-1）的受体关键性作用，这一研究结果为研制新的抗癌药物提供新的靶点。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with PAR1 polyclonal antibody, unconjugated (bs-0828R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAR1 Polyclonal Antibody, Unconjugated (bs-0828R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAR1 Polyclonal Antibody, Unconjugated (bs-0828R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAR1 Polyclonal Antibody, Unconjugated (bs-0828R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAR1 Polyclonal Antibody, Unconjugated (bs-0828R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAR1 Polyclonal Antibody, Unconjugated (bs-0828R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAR1 Polyclonal Antibody, Unconjugated (bs-0828R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
