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Rabbit Anti-PAF antibody (bs-1119R)
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说明书: 100ul  
100ul/1580.00元
大包装/询价

产品编号 bs-1119R
英文名称 PAF
中文名称 血小板活化因子抗体
别    名 platelet-activating factor; 1-Hexadecyl-2-acetyl-sn-glycero-3-phosphocholine; 1-O-Palmityl-2-acetyl-sn-glycero-3-phosphocholine monohydrate; 2-Acetyl-1-hexadecyl-sn-glycero-3-phosphocholine; 3-sn-Phosphatidylcholine; 1-hexadecyl-2-acetyl, PAF(C16.  
规格价格 100ul/1580元 购买        大包装/询价
说 明 书 100ul  
产品类型 药物与化合物抗体 
研究领域 药物及化合物  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Rat, PAF
产品应用 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 0.524kDa
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated PAF: 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
Platelet-activating factor, a compound that reduces inflammation by increasing permeability of blood vessels and contracting various involuntary muscles such as those in airways. It is a phospholipid with a wide range of proinflammatory actions, is immediately degraded and inactivated in vivo by PAF acetylhydrolase.

SWISS:
N/A

CAS:
74389-68-7

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

血小板活化因子(platelet-activating factor,PAF)是一种内源性具有生物活性的磷脂。由中性粒细胞、血小板、肥大细胞、内皮细胞和巨噬细胞产生。除早期发现它有强烈引起血小板聚集和脱颗粒作用外,近年来的研究发现它还具有广泛的生物活性,作为一种独特的细胞因子参与体内多种生理、病理过程,包括激活中性粒细胞,使其聚集、趋化、释放氧自由基和白三烯,抑制T细胞增殖,刺激B细胞合成IgG和IgE,抑制心肌收缩力,增加血管通透性,引起体循环血压下降和肺动脉高压等。由于它具有广泛的高强度的生物活性,故PAF被认为是与过敏反应有关的关键性介质之一。
产品图片
Tissue/cell: rat colon tissue;4% Paraformaldehyde-fixed and paraffin-embedded
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min
Incubation: Anti-PAF Polyclonal Antibody, Unconjugated(bs-1119R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
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