| 产品编号 | bs-0197R |
| 英文名称 | Rabbit Anti-VIPR2 antibody |
| 中文名称 | 腺苷酸环化酶激活肽受体-II/血管活性肠肽受体-II抗体 |
| 别 名 | VIP receptor II; VIP receptor2; VIP receptor-2; PACAP type-3; VIP type-2 receptor; Vip2; VPAC2; VPAC2R; Vasoactive intestinal polypeptide receptor 2; VIP-R-2; Pituitary adenylate cyclase-activating polypeptide type III receptor; PACAP type III receptor; PACAP-R-3; PACAP-R3; VIPR2_HUMAN. VIP-R2; VIP-2R |
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Specific References (3) | bs-0197R has been referenced in 3 publications.
[IF=3.188] Zeqi Tang. et al. Seasonal changes in the expression of PACAP, VPAC1, VPAC2, PAC1 and testicular activity in the testis of the muskrat (Ondatra zibethicus). EUR J HISTOCHEM. 2022 Mar 24; 66(2): 3398 IHC ; Rat.
[IF=2.454] Ye D et al. Spatiotemporal Expression Changes of PACAP and Its Receptors in Retinal Ganglion Cells After Optic Nerve Crush.(2018)J Mol Neurosci. Nov 10. WB&IF ; Rat.
[IF=2.22] Ruan, Ming, et al. "Attenuation of stress-induced gastrointestinal motility disorder by gentiopicroside, from Gentiana macrophylla Pall." Fitoterapia(2015). WB ; Rat.
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| 研究领域 | 细胞生物 免疫学 神经生物学 信号转导 生长因子和激素 细胞膜受体 G蛋白偶联受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=2ug/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 48kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from rat VIP receptor II: 81-170/437 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide belonging to the vasoactive intestinal polypeptide/glucagon/ secretin family. It is widely distributed in the body, and a variety of biological actions have been reported. Recent studies have shown that there is a family of PACAP receptors (PACAPRs), and two members of this family have been identified. Mouse PACAPR-3 is a protein of 437 amino acids that has 50% and 51% identity with rat PACAP type I and type II receptors, respectively. It binds to vasoactive intestinal polypeptide as well as PACAP-38 and -27, with a slightly higher affinity for PACAP-38, PACAPR-3 mRNA is expressed at high levels in MIN6, at moderate levels in pancreatic islets and other insulin-secreting cell lines, HIT-T15 and RINm5F, as well as in the lung, brain, stomach, and colon, and at low levels in the heart. PACAPR-3 participates in the regulation of insulin secretion. insulin secretion from MIN6 cells is significantly stimulated by PACAP-38. Function: This is a receptor for VIP as well as PACAP-38 and -27, the activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Can be coupled to phospholipase C. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in CD4+ T-cells, but not in CD8+ T-cells. Expressed in the T-cell lines Jurkat, PEER, MOLT-4, HSB, YT and Tsup-1, but not in the T-cell lines HARRIS and HUT 78. Similarity: Belongs to the G-protein coupled receptor 2 family. SWISS: P35000 Gene ID: 7434 Database links: Entrez Gene: 7434 Human Omim: 601970 Human SwissProt: P41587 Human Unigene: 585052 Human PACAP 受体分为两个亚型，PACAP-2型受体对PACAP是特异性的，与PACAP的亲和力比VIP高100-1000倍，PACAP-II型受体与PACAP和VIP的亲和力相似，对二者无选择性。此抗体即为后者。 |
| 产品图片 |
Sample: A431 Cell Lysate at 40 ug
Primary: Anti- VPAC2R (bs-0197R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 63 kD
Sample:
Lane 1: Recombinant human VIPR2 protein, N-Trx-His(bs-42334P)
Primary: Anti-VIPR2 (bs-0197R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 34 kDa
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-VIP receptor 2/VPAC2 Polyclonal Antibody, Unconjugated(bs-0197R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-VPAC2R antibody (bs-0197R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 2μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
