| 产品编号 | bs-0427R |
| 英文名称 | Rabbit Anti-P311 antibody |
| 中文名称 | 神经再生相关蛋白抗体 |
| 别 名 | NREP; Neuronal regeneration-related protein; C5orf13; Neuronal protein 3.1; PRO1873; Protein p311; P311 protein; Chromosome 5 open reading frame 13; D4S114; PTZ17; NREP_HUMAN. |
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Specific References (7) | bs-0427R has been referenced in 7 publications.
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| 研究领域 | 肿瘤 心血管 细胞生物 神经生物学 细胞周期蛋白 细胞分化 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Human,Rat) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 7.9kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human P311: 25-68/68 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
P311, also called PTZ17, was identified by suppressive subtraction hybridization as potentially involved in smooth muscle (SM) myogenesis., an 8-kDa polypeptide, was previously shown to be highly expressed in invasive glioma cells. P311 is constitutively serine-phosphorylated; decreased phosphorylation is observed in migration-activated glioma cells. The primary amino acid sequence of P311 indicates a putative serine phosphorylation site (S59) near the PEST domain. Site-directed mutagenesis of S59A retarded P311 degradation and induced glioma cell motility. In contrast, S59D mutation resulted in the rapid degradation of P311 and reduced glioma cell migration. Function: May have roles in neural function. Ectopic expression augments motility of gliomas. Promotes also axonal regeneration (By similarity). May also have functions in cellular differentiation (By similarity). Induces differentiation of fibroblast into myofibroblast and myofibroblast ameboid migration. Increases retinoic-acid regulation of lipid-droplet biogenesis (By similarity). Down-regulates the expression of TGFB1 and TGFB2 but not of TGFB3 (By similarity). May play a role in the regulation of alveolar generation. Subunit: Interacts with the latency-associated peptides (LAP) of TGFB1 and TGFB2; the interaction results in a decrease in TGFB autoinduction (By similarity). Interacts with FLNA. Subcellular Location: Cytoplasm. Tissue Specificity: Expressed in lung (at protein level). Post-translational modifications: Phosphorylated on Ser-59. Phosphorylation decreases stability and activity. SWISS: Q16612 Gene ID: 9315 Database links: Entrez Gene: 9315 Human Entrez Gene: 27528 Mouse Omim: 607332 Human SwissProt: Q16612 Human SwissProt: Q07475 Mouse Unigene: 36053 Human Unigene: 694860 Human Unigene: 728773 Human Unigene: 407415 Mouse Unigene: 8180 Rat P311 protein 还称：PTZ17 (pentylenetetrazd117)又称：增生性瘢痕相关蛋白。该蛋白与烧伤后瘢痕增生修复有关。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
