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Oct4 Rabbit pAb (bs-0830R)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
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产品编号 bs-0830R
英文名称 Oct4 Rabbit pAb
中文名称 胚胎干细胞关键蛋白抗体
别    名 MGC22487; Oct 3; Oct 4; Oct-3; Oct-4; OCT3; Oct4; Octamer binding protein 3; Octamer-binding protein 3; Octamer binding protein 4; Octamer binding transcription factor 3; Octamer-binding transcription factor 3; Octamer-4; POU domain transcription factor OCT4; OTF3; OTF4; OTF 3; OTF 4; OTF-3; POU class 5 homeobox 1; POU domain, class 5, transcription factor 1; POU domain class 5 transcription factor 1; PO5F1_HUMAN; POU type homeodomain containing DNA binding protein; POU5F1.  
Specific References  (14)     |     bs-0830R has been referenced in 14 publications.
[IF=8.755] Sijie Wang. et al. PFKFB4 facilitates palbociclib resistance in oestrogen receptor-positive breast cancer by enhancing stemness. CELL PROLIFERAT. 2022 Sep;:e13337  WB ;  Human.  
[IF=8.469] Que, Tianshi. et al. HMGA1 stimulates MYH9-dependent ubiquitination of GSK-3β via PI3K/Akt/c-Jun signaling to promote malignant progression and chemoresistance in gliomas. Cell Death Dis. 2021 Dec;12(12):1-12  WB ;  Human.  
[IF=6.217] Wang L et al. Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/2 and Akt pathways. J Exp Clin Cancer Res. 2019 Feb 21;38(1):93.  IF&WB ;  Human.  
[IF=3.929] Chen Z et al. Generation of an iPSC line (SKLOi001-A) from a patient with CLCN2-related leukoencephalopathy. Stem Cell Res. 2020 Mar 17;45:101769.  ICC ;  Human.  
[IF=3.905] Xiong Xiao. et al. Construction of extracellular matrix-based 3D hydrogel and its effects on cardiomyocytes. Exp Cell Res. 2021 Nov;408:112843  IHC ;  Mouse.  
[IF=3.811] Sun Z et al. Glioblastoma Stem Cell-Derived Exosomes Enhance Stemness and Tumorigenicity of Glioma Cells by Transferring Notch1 Protein. Cell Mol Neurobiol. 2019 Dec 18.  WB ;  Human&Mouse.  
[IF=3.44] Li X et al. A comparative study of the behavior of neural progenitor cells in extrusion-based in vitro hydrogel models. Biomed Mater. 2019 Sep 9;14(6):065001.  WB ;  Mouse.  
[IF=3.225] Lin Li. et al. Breast cancer stem cells-derived extracellular vesicles affect PPARG expression by delivering microRNA-197 in breast cancer cells. Clin Breast Cancer. 2022 Feb;:  IF ;  Human.  
[IF=3.182] Liu K et al. Verteporfin suppresses the proliferation, epithelial-mesenchymal transition and stemness of head and neck squamous carcinoma cells via inhibiting YAP1. Journal of Cancer. 2019; 10(18): 4196-4207.  WB ;  Human.  
[IF=2.93] Tang, Xin, et al. "Downregulation of CXCR7 inhibits proliferative capacity and stem cell-like properties in breast cancer stem cells." Tumor Biology (2016): 1-9.  WB ;  Human.  
[IF=2.634] Ma C et al. Identification and Multilineage Potential Research of a Novel Type of Adipose-Derived Mesenchymal Stem Cells from Goose Inguinal Groove. DNA Cell Biol. 2018 Sep;37(9):731-741.  ICF ;  Goose.  
[IF=2.479] Wenjia Fang. et al. Prognostic value of OCT4 in colorectal cancer: analysis using immunohistochemistry and bioinformatics validation. 2020 Oct 14  IHC ;  
[IF=1.587] Ping Xu. et al. Generation and characterization of two iPSC lines carrying heterozygous or homozygous nonsense mutation in PROM1 gene from a single family. STEM CELL RES. 2022 Sep;:102913  WB ;  Human.  
[IF=1.461] Pei W et al. Biological characterization and pluripotent identification of ovine amniotic fluid stem cells. Cytotechnology. 2018 Jun;70(3):1009-1021.  IF&FCM ;  ovine embryo.  
研究领域 细胞生物  染色质和核信号  信号转导  干细胞  细胞周期蛋白  转录调节因子  细胞类型标志物  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse,Rat (predicted: Pig,Cow,Dog)
产品应用 WB=1:500-2000,Flow-Cyt=1ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 39 kDa
检测分子量
细胞定位 细胞核 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human OCT4: 201-300/360 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.

Function:
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

Subunit:
Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2.

Subcellular Location:
Nucleus.Note=Expressed in a diffuse and slightly punctuate pattern.

Tissue Specificity:
Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.

Post-translational modifications:
Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression (By similarity).
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation (By similarity).
ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.

Similarity:
Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.

SWISS:
Q01860

Gene ID:
5460

Database links:

Entrez Gene: 282316 Cow

Entrez Gene: 5460 Human

Entrez Gene: 18999 Mouse

Entrez Gene: 100127461 Pig

Entrez Gene: 294562 Rat

Omim: 164177 Human

SwissProt: O97552 Cow

SwissProt: Q01860 Human

SwissProt: P20263 Mouse

SwissProt: Q9TSV5 Pig

Unigene: 249184 Human

Unigene: 632482 Human

Unigene: 646545 Human

Unigene: 17031 Mouse

Unigene: 161748 Rat



产品图片
Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Heart (Mouse) Lysate at 40 ug Lane 3: Cerebellum (Mouse) Lysate at 40 ug Lane 4: Testis (Mouse) Lysate at 40 ug Lane 5: Cerebrum (Rat) Lysate at 40 ug Lane 6: Heart (Rat) Lysate at 40 ug Lan
Blank control: Hela. Primary Antibody (green line): Rabbit Anti-4-Oct antibody (bs-0830R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol T
Blank control (Black line): Mouse spleen (Black). Primary Antibody (green line): Rabbit Anti-Oct4 antibody (bs-0830R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rab
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