| 产品编号 | bs-1077R |
| 英文名称 | Rabbit Anti-OCT2 antibody |
| 中文名称 | 阳离子转运蛋白2抗体 |
| 别 名 | organic cation transporter OCT2; POU domain, class 2, transcription factor 2; Octamer-binding transcription factor 2; Oct-2; OTF-2; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; POU2F2; NF A2; Oct 2; PO2F2_MOUSE; PO2F2_HUMAN. |
 |
Specific References (3) | bs-1077R has been referenced in 3 publications.
[IF=10.273] Singh NK et al. Three-dimensional cell-printing of advanced renal tubular tissue analogue. Biomaterials. 2019 Dec 26;232:119734. WB&IHF-P ; Mouse.
[IF=3.3] Xu C, Zhu L, Chan T, Lu X, Shen W, et al. (2015) The Altered Renal and Hepatic Expression of Solute Carrier Transporters (SLCs) in Type 1 Diabetic Mice. PLoS ONE 10(3): e0120760. doi:10.1371/journal.pone.0120760 WB ; Mouse.
[IF=2.31] Zhou LN et al. Macrophage polarization in human gingival tissue in response to periodontal disease.(2018) Oral Dis. Oct 4. IHC ; Human.
|
| 研究领域 | 细胞生物 免疫学 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 51kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse OCT2: 11-100/463 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Oct2 is a transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Oct2 regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. It also modulates transcription transactivation by NR3C1, AR and PGR. Oct2 is B cell specific, belongs to the POU transcription factor family class 2 subfamily and contains 1 homeobox domain. Function: Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter. Subunit: Interacts with NR3C1, AR and PGR. Subcellular Location: Cytoplasm. Nuclear Tissue Specificity: Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line Molt-4, but not in the T-cell line BW 5147. Similarity: Belongs to the POU transcription factor family. Class-2 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain. SWISS: Q00196 Gene ID: 18987 Database links: Entrez Gene: 5452 Human Entrez Gene: 18987 Mouse Omim: 164176 Human SwissProt: P09086 Human SwissProt: Q00196 Mouse Unigene: 147381 Human Unigene: 76683 Mouse Unigene: 155998 Rat 同源结构域蛋白（Homeodomain Proteins） OCT-2是B淋巴细胞转录因子，以免疫球蛋白启动子为目标，于生发中心形成和B细胞分化成浆细胞中起作用。 |
| 产品图片 |
Sample:
A549 Cell lysate at 30 ug;
Primary: Anti-OCT2 (bs-1077R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution;
Predicted band size : 51 kD
Observed band size : 51 kD
Sample:
Lane 1: Mouse Lymph node tissue lysates
Lane 2: Rat Lymph node tissue lysates
Primary: Anti-OCT2 (bs-1077R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-OCT2 Polyclonal Antibody, Unconjugated(bs-1077R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-OCT2 Polyclonal Antibody, Unconjugated(bs-1077R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
