| 产品编号 | bs-0158R |
| 英文名称 | Neurotrophin 4 Rabbit pAb |
| 中文名称 | 神经生长因子4/5抗体 |
| 别 名 | GLC1O; Neurotrophic factor 4; Neurotrophic factor 5; Neurotrophin 4/5; Neurotrophin 5(neurotrophin 4/5); Neurotrophin 5; Neurotrophin-4; Neurotrophin-5; Neurotrophin4; Neurotrophin4/5; Neurotrophin5; Neutrophic factor 4; Neutrophic factor 5; NT 4; NT-4; NT 4/5; NT-4/5; NT 5; NT-5; NT4; NT4/5; NT4P; NT5; NTF 4; NTF 5; NTF4; NTF4_HUMAN; NTF5 |
| 研究领域 | 细胞生物 免疫学 神经生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Rat (predicted: Mouse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 14/27 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NT-4: 121-210/210 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Neurotrophin 4 (NT4; synonymous with NT5) belongs to the NGF beta family and is a survival factor for peripheral sensory sympathetic neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. NT4 levels are highest in the prostate, with lower levels in thymus, placenta, and skeletal muscle. NT4 is also expressed in embryonic and adult tissues.
Function: Target-derived survival factor for peripheral sensory sympathetic neurons. Subcellular Location: Secreted. Tissue Specificity: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. DISEASE: Defects in NTF4 may be associated with susceptibility to primary open angle glaucoma type 1O (GLC1O) [MIM:613100]. A form of primary open angle glaucoma (POAG). POAG is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place. Similarity: Belongs to the NGF-beta family. SWISS: P34130 Gene ID: 4909 Database links: Entrez Gene: 4909 Human Entrez Gene: 78405 Mouse Omim: 162662 Human SwissProt: P34130 Human SwissProt: Q80VU4 Mouse Unigene: 266902 Human Unigene: 20344 Mouse Unigene: 44225 Rat Neurotrophic factor 4/5起源于同一基因家族， 故可称为NGF家族。神经生长因子4/5具有促进早期神经元的增殖与分化，维持成熟神经元的存活的生物学功能,能调控神经元再生的微环境、可促进运动神经元的存活和生长并能防止它受损及死亡。 |
| 产品图片 |
Sample: DU145 Cell (Human) Lysate at 40 ug
Primary: Anti-Neurotrophin 4 (bs-0158R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 14/27 kD
Observed band size: 26 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Neurotrophin 4/NT-4/NT-5 Polyclonal Antibody, Unconjugated(bs-0158R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
