| 产品编号 | bs-0075R |
| 英文名称 | Nociceptin Rabbit pAb |
| 中文名称 | 孤菲肽/痛敏肽抗体 |
| 别 名 | PNOC; N/OFQ; N23K; N23K/N27K; Nociceptin, included; Npnc1; OFQ; OFQ/N; Orphanin FQ; PPNOC; prepronociceptin; Propronociceptin; PNOC_HUMAN. |
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Specific References (1) | bs-0075R has been referenced in 1 publications.
[IF=1.931] Lyu J et al. Botulinum toxin A alleviates orofacial nociception induced by orthodontic tooth movement through nociceptin/orphanin-FQ pathway in rats. Arch Oral Biol
. 2020 Sep;117:104817. WB ; Rat.
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| 研究领域 | 肿瘤 心血管 神经生物学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat (predicted: Human,Mouse,Rabbit,Pig,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 20 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nociceptin: 130-146/176 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Nociceptin (PNOC) is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development. Expressed predominantly in the spinal cord and brain, being more abundant in the hypothalamus and striatum. Also found in small amounts in ovary. Belongs to the opioid neuropeptide precursor family. Function: Nociceptin is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development (By similarity). Subcellular Location: Secreted. Tissue Specificity: Predominantly in the brain and spinal cord. Similarity: Belongs to the opioid neuropeptide precursor family. SWISS: Q13519 Gene ID: 5368 Database links: Entrez Gene: 5368 Human Entrez Gene: 18155 Mouse Omim: 601459 Human SwissProt: Q13519 Human SwissProt: Q64387 Mouse Unigene: 88218 Human Unigene: 16347 Mouse Unigene: 486163 Mouse Unigene: 87935 Rat 孤菲肽（Nociceptin又称为Orphanin FQ; PPNOC; ORL1）是新发现的一种神经肽，由17个氨基酸组成，又称痛敏肽。消化道肿瘤病人的血浆中OFQ含量明显高于对照组，尤以结肠癌、胃癌最为明显。 |
| 产品图片 |
Sample:
Brain(Rat) lysate at 30ug;
Liver(Rat) lysate at 30ug;
Primary: Anti-Nociceptin(bs-0075R) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 20kD
Observed band size : 20kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Nociceptin Polyclonal Antibody, Unconjugated(bs-0075R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
