[IF=3.706] Shoko Yoshida. et al. Expression of Neurokinin B Receptor in the Gingival Squamous Cell Carcinoma Bone Microenvironment. Diagnostics. 2021 Jun;11(6):1044  IHC ;  Human.  

PubMed:34200131

[IF=1.9] OBATA, KYOICHI, et al. "Role of Neurokinin 3 Receptor Signaling in Oral Squamous Cell Carcinoma." Anticancer Research 37.11 (2017): 6119-6123.  IHC-P ;  Mouse.  

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[IF=1.83] OBATA, KYOICHI, et al. "Tachykinin Receptor 3 Distribution in Human Oral Squamous Cell Carcinoma." Anticancer Research 36.12 (2016): 6335-6341.  IHC-P ;  Human.  

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Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Neurokin B receptor) Polyclonal Antibody, Unconjugated (bs-0166R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: Mouse lung tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NK3R Polyclonal Antibody, Unconjugated(bs-0166R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei

Blank control: RSC96(blue).
Primary Antibody: Rabbit Anti- Neurokin B receptor antibody(bs-0166R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
Primary antibody (bs-0166R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.