| 产品编号 | bs-0448R |
| 英文名称 | Rabbit Anti-NIS antibody |
| 中文名称 | 钠碘转运体蛋白抗体 |
| 别 名 | Na(+)/I(-) cotransporter; Na(+)/I(-) symporter; Na(+)/I(-) cotransporter; Na(+)/I(-) symporter; Na+/I- cotransporter; Na+/I-symporter; NIS; SC5A5_HUMAN; SLC5A5; sodium iodide symporter; Sodium-iodide symporter; Sodium/iodide cotransporter; Solute carrier family 5 (sodium iodide symporter) member 5; Solute carrier family 5; Solute carrier family 5 member 5. |
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Specific References (11) | bs-0448R has been referenced in 11 publications.
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| 研究领域 | 免疫学 通道蛋白 转运蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 68kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NIS: 525-618/618 <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
catalyzes Na+/I- symporter activity plays a role in iodide transport and thyroid hormone generation. Human Sodium Iodide Symporter (hNIS) is responsible for iodide concentrating ability within thyroid follicular cells. It is a membrane bound glycoprotein with 13 membrane spanning domains and 14 extramembranous domains. It may represent an autoantigen in thyroid. Function: Mediates iodide uptake in the thyroid gland. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Expression is primarily in thyroid tissue, but also to a lower extent in mammary gland and ovary. Expression is reduced in tumors. DISEASE: Thyroid dyshormonogenesis 1 (TDH1) [MIM:274400]: A disorder characterized by the inability of the thyroid to maintain a concentration difference of readily exchangeable iodine between the plasma and the thyroid gland, leading to congenital hypothyroidism. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. SWISS: Q99PN0 Gene ID: 6528 Database links: Entrez Gene: 6528 Human Omim: 601843 Human SwissProt: Q92911 Human Unigene: 103983 Human Unigene: 584804 Human Unigene: 10505 Rat 钠/碘转运体蛋白-NIS，是位于甲状腺细胞基底膜上的一类膜抗原，在甲状腺对碘的主动转运过程中发挥重要作用。 钠/碘转运蛋白受甲状腺刺激素(TSH)、碘、细胞因子和生长因子等多种因素的广泛调节。自身免疫性甲状腺疾病时NIS的表达发生了明显变化，患者体内出现抗NIS自身抗体。 此外，NIS还与甲状腺冷结节和先天性甲状腺机能减退等甲状腺疾病的发病机理密切相关，近年来得到了足够的重视。 |
| 产品图片 |
Sample:
Lane 1: Mouse Stomach tissue lysates
Lane 2: Rat Stomach tissue lysates
Primary: Anti-NIS (bs-0448R) at 1/200 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 48 kDa
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NIS Polyclonal Antibody, Unconjugated(bs-0448R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat thyroid gland; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NIS Polyclonal Antibody, Unconjugated(bs-0448R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
