| 产品编号 | bs-0505R |
| 英文名称 | Rabbit Anti-Sodium hydrogen exchanger 1 antibody |
| 中文名称 | 钠氢通道蛋白抗体 |
| 别 名 | amiloride-sensitive; APNH; APNH1; FLJ42224; Na Li countertransporter; Na(+)/H(+) antiporter; Na(+)/H(+) exchanger 1; Na+ H+ antiporter amiloride-sensitive; Na+ H+ antiporter; Na+ H+ exchanger 1; NHE-1; NHE1; OTTHUMP00000004468; SL9A1_HUMAN; SLC9A1; Sodium hydrogen exchanger 1; Sodium/hydrogen exchanger 1; solute carrier family 9; Solute carrier family 9 member 1; Solute carrier family 9 sodium hydrogen exchanger isoform 1 antiporter Na+ H+ amiloride sensitive; Solute carrier family 9 subfamily A (NHE1 cation proton antiporter 1) member 1; Solute carrier family 9 subfamily A member 1. |
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Specific References (2) | bs-0505R has been referenced in 2 publications.
[IF=7.675] Ya-Ling Yin. et al. Citronellal Attenuates Oxidative Stress–Induced Mitochondrial Damage through TRPM2/NHE1 Pathway and Effectively Inhibits Endothelial Dysfunction in Type 2 Diabetes Mellitus. ANTIOXIDANTS-BASEL. 2022 Nov;11(11):2241 IHC, IF ; Rat, Human.
[IF=5.714] Yaling Yin. et al. PAE ameliorates doxorubicin-induced cardiotoxicity via suppressing NHE1 phosphorylation and stimulating PI3K/AKT phosphorylation. INT IMMUNOPHARMACOL. 2022 Dec;113:109274 IHC ; Rat.
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| 研究领域 | 心血管 细胞生物 信号转导 通道蛋白 转运蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 91kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NHE1: 470-550/815 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a Na+/H+ antiporter that is a member of the solute carrier family 9. The encoded protein is a plasma membrane transporter that is expressed in the kidney and intestine. This protein plays a central role in regulating pH homeostasis, cell migration and cell volume. This protein may also be involved in tumor growth. [provided by RefSeq, Sep 2011] Function: Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. Subunit: Oligomer. Interacts with calmodulin and TESC. Interacts (via the juxtamembrane region of the cytoplasmic C-terminus domain) with CHP1; the interaction occurs at the plasma membrane in a calcium-dependent manner. Interacts with CHP2; the interaction occurs in a calcium-dependent manner. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Kidney and intestine. Post-translational modifications: O-glycosylated. Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is reduced by CHP1. Similarity: Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. SWISS: P19634 Gene ID: 6548 Database links: Entrez Gene: 6548 Human Entrez Gene: 20544 Mouse Omim: 107310 Human SwissProt: P19634 Human SwissProt: Q61165 Mouse Unigene: 469116 Human Unigene: 4312 Mouse Unigene: 5025 Rat 通道蛋白（Channel Protein） 钠-氢交换蛋白（NHE： Na+/H+ Exchanger）是维持细胞内环境的主要细胞膜蛋白质之一，它的一个主要功能是调节细胞内pH值，使之保持在生理范围之内。细胞内pH值的变化，直接或间接的影响细胞的生长分裂、增殖和分化，也影响细胞的死亡。 钠-氢交换蛋白质的活性还影响细胞的移动和肿瘤细胞的转移。 目前主要方向是：研究消化道肿瘤细胞转移。 |
| 产品图片 |
Sample:
Brain(Rat) lysate at 30ug;
Liver(Rat) lysate at 30ug;
Primary: Anti-NHE1 (bs-0505R) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution;
Predicted band size : 91kD
Observed band size : 91kD
We are unsure as to the identity of these extra bands
Sample:
molt-4(human)cell Lysate at 40 ug
Primary: Anti- Sodium hydrogen exchanger 1 (bs-0505R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 91kD
Observed band size: 91 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Sodium hydrogen exchanger 1) Polyclonal Antibody, Unconjugated (bs-0505R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
