| 产品编号 | bs-0423R |
| 英文名称 | MMP7 Rabbit pAb |
| 中文名称 | 基质金属蛋白酶-7抗体 |
| 别 名 | Matrilysin; Matrilysin uterine; Matrin; Matrix Metalloproteinase 7; MMP-7; MMP 7; MMP7; MPSL1; PUMP 1; Pump 1 protease; PUMP1; Uterine Matrilysin; Uterine metalloproteinase; MMP7_HUMAN. |
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Specific References (10) | bs-0423R has been referenced in 10 publications.
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| 研究领域 | 肿瘤 免疫学 神经生物学 细胞凋亡 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,ELISA=1:2000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 30 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MMP7: 151-250/269 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal protein domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008] Function: Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase. Subcellular Location: Secreted, extracellular space, extracellular matrix (Probable). Similarity: Belongs to the peptidase M10A family. SWISS: P09237 Gene ID: 4316 Database links: Entrez Gene: 4316 Human Entrez Gene: 17393 Mouse Omim: 178990 Human SwissProt: P09237 Human SwissProt: Q10738 Mouse Unigene: 2256 Human Unigene: 4825 Mouse Unigene: 10282 Rat 合成与降解（Synthesis and Degradation） MMP7属于MMP基因家族的成员之一，MMP-7又称溶基质蛋白素(Matrilysin)，其主要功能为降解粘蛋白、纤维连结蛋白和层粘连蛋白，在肿瘤细胞突破基底膜屏障和浸润、转移中起重要作用。目前主要用于各种恶性肿瘤(如乳腺癌、胃肠道癌、卵巢癌、膀胱癌等)转移、浸润的研究及用于机体各种组织的发育和修复、肿瘤发生发展、炎症反应等过程中作用的研究。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with MMP7 polyclonal antibody, unconjugated (bs-0423R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Endometrium; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP7 Polyclonal Antibody, Unconjugated (bs-0423R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP7 Polyclonal Antibody, Unconjugated (bs-0423R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
