| 产品编号 | bs-0531R |
| 英文名称 | Rabbit Anti-Matriptase antibody |
| 中文名称 | 蛋白裂解酶（一种新的癌基因）抗体 |
| 别 名 | transmembrane protease, serine 6; 1300008A22Rik; IRIDA; MATRIPTASE2; TMPRSS6. |
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Specific References (1) | bs-0531R has been referenced in 1 publications.
[IF=2.608] Kim S et al. Capsaicin induces atopic dermatitis-like manifestations through dysregulation of proteolytic system and alteration of filaggrin processing in rats. Exp Dermatol. 2018 Apr;27(4):332-339. WB ; Rat.
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| 研究领域 | 肿瘤 细胞生物 激酶和磷酸酶 合成与降解 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat) |
| 产品应用 | ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 94kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Matriptase: 11-100/855 <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is an epithelial-derived, integral membrane serine protease. This protease forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. This protease has been shown to cleave and activate hepatocyte growth factor/scattering factor, and urokinase plasminogen activator, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. The expression of this protease has been associated with breast, colon, prostate, and ovarian tumors, which implicates its role in cancer invasion, and metastasis. Subunit: Interacts with CDCP1. May interact with TMEFF1. Subcellular Location: Membrane; Single-pass type II membrane protein (Probable). Similarity: Belongs to the peptidase S1 family. Contains 2 CUB domains. Contains 4 LDL-receptor class A domains. Contains 1 peptidase S1 domain. SWISS: Q9Y5Y6 Gene ID: 164656 Database links: Entrez Gene: 164656 Human Entrez Gene: 71753 Mouse Omim: 609862 Human SwissProt: Q8IU80 Human SwissProt: Q9DBI0 Mouse Unigene: 370885 Human Unigene: 34056 Mouse Unigene: 214123 Rat 蛋白裂解酶是第一种已在分子水平上辨明的、与癌症相关的跨膜丝氨酸蛋白酶。他们的研究首次发现蛋白裂解酶可以诱发肿瘤。蛋白裂解酶是一种细胞膜蛋白，一直以来研究者们对这种蛋白裂解酶的致癌功能表示怀疑。 matriptase在多种上皮来源的肿瘤中均高水平表达，有学者认为：matriptase作为一种癌基因，为跨膜蛋白而且位于细胞表面，部分位于胞浆。 |
| 产品图片 |
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Matriptase) polyclonal Antibody, Unconjugated (bs-0531R) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
