| 产品编号 | bs-0257R |
| 英文名称 | MAG Rabbit pAb |
| 中文名称 | 髓鞘相关糖蛋白a/b抗体 |
| 别 名 | GMA; MAG; MAG_HUMAN; Myelin associated glycoprotein; Myelin-associated glycoprotein; S MAG; Siglec 4a; Siglec-4a; SIGLEC4A. |
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Specific References (3) | bs-0257R has been referenced in 3 publications.
[IF=1.27] Feng, Nianping, et al. "Transplantation of mesenchymal stem cells promotes the functional recovery of the central nervous system following cerebral ischemia by inhibiting myelin‑associated inhibitor expression and neural apoptosis." Experimental and Therapeutic Medicine. IHC-P ; Rat.
[IF=1.26] Zhang et al. MicroRNA-210 contributes to peripheral nerve regeneration through promoting the proliferation and migration of Schwann cells. (2017) Exp.Ther.Med. 14:2809-2816 WB ; Rat.
[IF=1.17] Ozgun-Acar, Ozden, et al. "Capparis ovata treatment suppresses inflammatory cytokine expression and ameliorates experimental allergic encephalomyelitis model of multiple sclerosis in C57BL/6 mice." Journal of Neuroimmunology (2016). IHC-P ; Mouse.
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| 研究领域 | 细胞生物 神经生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat) |
| 产品应用 | WB=1:500-2000,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 67 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MAG-a/b: 501-582/582 <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
MAG (myelin associated glycoprotein) is a Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha2,3-linked sialic acid. Isoform L-MAG is critical for the formation of myelin in the CNS, whereas isoform S-MAG is sufficient to maintain the integrity of myelin in PNS. Binds to RTN4R. single-pass type I membrane protein. Expressed by myelinating glial cells in the central and peripheral nervous system. Detected in oligodendrocyte processes before formation of compact myelin. Restricted to the periaxonal space after myelination. Isoform S-MAG is the predominant isoform in CNS and PNS of the adult. In CNS isoform L-MAG is the major form synthesized early in development, and it persists as a significant proportion of the MAG present in the adult. In the PNS isoform L-MAG is expressed at modest levels during development; it is absent in the adult. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. Function: Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid. Subunit: Binds to RTN4R. Subcellular Location: Membrane; Single-pass type I membrane protein. Similarity: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. Contains 4 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: P20916 Gene ID: 4099 Database links: Entrez Gene: 4099 Human Entrez Gene: 17136 Mouse Omim: 159460 Human SwissProt: P20916 Human SwissProt: P20917 Mouse Unigene: 643440 Human Unigene: 241355 Mouse Unigene: 87331 Rat 髓鞘相关糖蛋白a/b（MAG-a/b）又称：髓磷脂相关的球蛋白 髓鞘相关糖蛋白(myelin-associated glycoprotein, MAG)是免疫球蛋白超家族成员，它由中枢神经系统的少突胶质细胞和外周神经系统的施万细胞表达。 MAG定位于直接和轴突相接触的髓鞘膜的最里层，它通过介导胶质细胞与轴突的相互作用参与髓鞘的形成及其完整性的维持。同时MAG也是髓鞘来源的神经生长抑制因子的主要成分。在神经系统发育的不同阶段，MAG显示不同的功能:即发育期促进轴突生长，成熟期抑制轴突生长。其抑制作用主要由髓鞘来源的抑制分子的共同受体NgR介导，在神经营养因子受体p75NTR以及小GTP酶Rho等信号分子的共同参与下完成。 |
| 产品图片 |
Sample: U251 Cell Lysate at 30 ug
Primary: Anti-MAG (bs-0257R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size:67 kD
Observed band size:67 kD
Blank control:SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-MAG antibody (bs-0257R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
