| 产品编号 | bs-1177R |
| 英文名称 | Rabbit Anti-LINGO1 antibody |
| 中文名称 | Nogo受体反应蛋白抗体 |
| 别 名 | FLJ14594; LERN 1; Lingo-1; LERN1; Lingo1; Leucine rich repeat and Ig domain containing 1; Leucine rich repeat neuronal protein 1; Leucine rich repeat neuronal protein 6A; Leucine-rich repeat neuronal 6A; Lingo 1; LRR and Ig domain-containing Nogo Receptor interating protein; Lrrn 6a; Lrrn6a; Lrrn6a protein; MGC17422; Nogo Receptor interacting protein; UNQ201. |
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Specific References (1) | bs-1177R has been referenced in 1 publications.
[IF=3.171] Huang Y et al. Mechanism of delayed encephalopathy after acute carbon monoxide poisoning. Neural Regen Res
. 2020 Dec;15(12):2286-2295. IHC-P ; Rat.
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| 研究领域 | 发育生物学 神经生物学 信号转导 通道蛋白 细胞膜受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human,Pig,Cow,Chicken,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 70 kDa |
| 检测分子量 | 75-85 |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LINGO1: 521-620/620 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
LINGO-1 (LRR and Ig domain-containing Nogo Receptor interating protein) is a nervous system-specific LRR-Ig-containing protein with an important role in CNS biology. LINGO-1 was discovered in a sequence database search for human SLIT homologs that were selectively expressed in the brain. LINGO-1 is a transmembrane protein that is a component of the Nogo-66 receptor complex. It binds NgR1 and p75 and is an additional functional componenet of the NgR1/p75 signaling complex. Function: Functional component of the Nogo receptor signaling complex (RTN4R/NGFR) in RhoA activation responsible for some inhibition of axonal regeneration by myelin-associated factors. Is also an important negative regulator of oligodentrocyte differentiation and axonal myelination. Acts in conjunction with RTN4 and RTN4R in regulating neuronal precursor cell motility during cortical development. Subunit: Homotetramer. Forms ternary complex with RTN4R/NGFR and RTN4R/TNFRSF19. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed exclusively in the central nervous system. Highest level in the in amygdala, hippocampus, thalamus and cerebral cortex. In the rest of the brain a basal expression seems to be always present. Up-regulated in substantia nigra neurons from Parkinson disease patients. Post-translational modifications: N-glycosylated. Contains predominantly high-mannose glycans. Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 11 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain. Contains 1 LRRNT domain SWISS: Q96FE5 Gene ID: 84894 Database links: Entrez Gene: 415344 Chicken Entrez Gene: 84894 Human Entrez Gene: 235402 Mouse SwissProt: Q50L44 Chicken SwissProt: Q6NUK3 Human SwissProt: Q96FE5 Human SwissProt: Q9D1T0 Mouse 神经生物学相关蛋白（Neurobiology） LINGO-1是一种体内体外少突胶质细胞(Oligodendrocyte)分化和髓鞘(myelination)的负调控因子，神经元上的LINGO-1被证明参与调节中枢神经再生的抑制信号，而少突胶质细胞表达的LINGO-1分子参与负调节少突胶质细胞的髓鞘化过程，是一种体内体外少突胶质细胞(Oligodendrocyte)分化和髓鞘(myelination)的负调控因子。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Lingo-1) Polyclonal Antibody, Unconjugated (bs-1177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Lingo-1) Polyclonal Antibody, Unconjugated (bs-1177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
