| 产品编号 | bs-1193R |
| 英文名称 | Rabbit Anti-IL-23 antibody |
| 中文名称 | 白介素-23抗体 |
| 别 名 | Interleukin-23 subunit alpha; Il23a; IL 23; IL 23A; IL23P19; Interleukin 23 alpha subunit p19; JKA3 induced upon T cell activation; P19; SGRF; IL-23 subunit alpha; IL-23-A; IL-23p19; IL23; Interleukin 23 p19 subunit; Interleukin-23 subunit p19; JKA3 induced upon T cell activation; MGC79388; IL23A_HUMAN. |
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Specific References (8) | bs-1193R has been referenced in 8 publications.
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| 研究领域 | 肿瘤 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 21kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IL-23 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
IL23 is a heterodimeric cytokine consisting in two subunits : p40, shared with IL12 and a distinct one p19 (the IL23 subunit). Although IL12 activates naive T cells, the initial description of IL23 was the preferential action on memory T cells to increase IFNg production. In conjunction with IL6 and TGF-beta1, IL23 is necessary to maintain the Th1 lineage. IL23 plays an important role in the inflammatory response against infection, and is implicated in the development of cancerous tumors and auto immune diseases. Over expression of p19 correlates with inflammation in multiple organs and epithelial tissues including skin. Function: Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. Subcellular Location: Secreted. Secreted upon association with IL12B. Tissue Specificity: Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level). Similarity: Belongs to the IL-6 superfamily. SWISS: Q9NPF7 Gene ID: 51561 Database links: Entrez Gene: 51561 Human Entrez Gene: 83430 Mouse Omim: 605580 Human SwissProt: Q9NPF7 Human SwissProt: Q9EQ14 Mouse Unigene: 382212 Human Unigene: 98309 Human Unigene: 125482 Mouse IL23(Interleukin-23)作为一种前炎症细胞因子，具有影响机体免疫反应和抗感染作用,白介素23能连接肿瘤关联的炎症和肿瘤免疫监督缺乏关系的细胞因子。存在于很多人类肿瘤中，能通过促进血管形成来帮助肿瘤生长。 |
| 产品图片 |
Sample:
Lane 1: Mouse Ear tissue lysates
Lane 2: Human A431 cell lysates
Lane 3: Human Jurkat cell lysates
Primary: Anti-IL-23 (bs-1193R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Tissue/cell: human rhinopolypus tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IL-23 Polyclonal Antibody, Unconjugated(bs-1193R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
