| 产品编号 | bs-0214R |
| 英文名称 | Rabbit Anti-DFFA antibody |
| 中文名称 | DFFA抗体 |
| 别 名 | DNA fragmentation factor subunit alpha; DFF-45; Inhibitor of CAD; ICAD; DFF1; DFF45; H13; DFFA; DFFA_HUMAN; |
| 研究领域 | 细胞生物 免疫学 染色质和核信号 细胞凋亡 新陈代谢 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 36kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ICAD: 2-100/331 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Caspase-activated deoxyribonuclease(CAD) also designated DNA fragmentation factor 45 kDa subunit (DFF-45)and Caspase-activated DNase. CAD is a nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. ICAD (DNA fragmentation factor alpha subunit) (DNA fragmentation factor 45 kDa subunit) (DFF-45) (Inhibitor of CAD) is inhibitor of the caspase-activated DNase (DFF44). Function: Inhibitor of the caspase-activated DNase (DFF40). Subunit: Heterodimer of DFFA and DFFB. Subcellular Location: Cytoplasm. Post-translational modifications: Caspase-3 cleaves DFF45 at 2 sites to generate an active factor. Similarity: Contains 1 CIDE-N domain. SWISS: O54786 Gene ID: 1676 Database links: Entrez Gene: 1676 Human Omim: 601882 Human SwissProt: O00273 Human Unigene: 484782 Human ICAD 是在细胞凋亡期诱导DNA分裂和染色体固缩的一种核酶，降解裸露的DNA 并诱导形态学变化。 |
| 产品图片 |
Sample:
liver (mouse) Lysate at 40 ug
lung(mouse) Lysate at 40 ug
BRL-3A (rat)cell Lysate at 40 ug
Primary: Anti- ICAD (bs-0214R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36kD
Observed band size: 36 kD
Sample: A431 Cell (Human) Lysate at 40 ug
Primary: Anti-ICAD (bs-0214R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD
Tissue/cell: Human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ICAD Polyclonal Antibody, Unconjugated(bs-0214R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ICAD/DFF-45 Beta Polyclonal Antibody, Unconjugated(bs-0214R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
