| 产品编号 | bs-0752R |
| 英文名称 | HLA G Rabbit pAb |
| 中文名称 | 人类白细胞抗原G抗体 |
| 别 名 | MHC-G; HLAG_HUMAN; HLA-G; HLA G antigen; MHC class I antigen G; HLA-6.0; HLAG; |
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Specific References (2) | bs-0752R has been referenced in 2 publications.
[IF=3.762] Sanchez-Ruiz M et al. Enteric murine ganglionitis induced by autoimmune CD8 T cells mimics human gastrointestinal dysmotility. Am J Pathol. 2019 Mar;189(3):540-551. IHC&IHF ; Human.
[IF=1.894] Tan H et al. Is Atrial Natriuretic Peptide (ANP) and Natriuretic Peptide Receptor-A (NPR-A) Expression in Human Placenta and Decidua Normal? Med Sci Monit. 2019 Apr 19;25:2868-2878. IHC-P ; Human.
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| 研究领域 | 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Rat |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 35 kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from the middle of human HLA-G: 181-280/338 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008] Subunit: Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin). Homodimer; disulfide-linked. Binds to LILRB1 and LILRB2. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed in trophoblasts. Similarity: Belongs to the MHC class I family. Contains 1 Ig-like C1-type (immunoglobulin-like) SWISS: P17693 Gene ID: 3135 Database links: Entrez Gene: 3135 Human Omim: 142871 Human SwissProt: P17693 Human Unigene: 512152 Human 人类白细胞抗原G/组织相容性白细胞抗原G(Human Leukocyte Antigen-G, HLA-G )是一种非典型的白细胞抗原Ⅰ族组织相溶性抗原。该抗原在正常情况下仅在胎盘的绒毛膜表达。在病理情况下HLA-G只在某些癌细胞表达，在正常组织或良性肿瘤则很少表达.因此, HLA-G是一个具有高度肿瘤特异性的肿瘤标志物。 近年研究认为HLA—G作为免疫耐受分子，在肿瘤免疫逃逸中可能具有重要作用. |
| 产品图片 |
Sample:
Brain (Rat) Lysate at 30 ug
Kidney (Rat) lysate at 30 ug
Primary: Anti- HLA G (bs-0752R) at 1/200 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/3000 dilution
Predicted band size: 35 kD
Observed band size: 35 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HLA B/HLA G Polyclonal Antibody, Unconjugated(bs-0752R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
