产品编号 | bs-0287R |
英文名称 | His tag |
中文名称 | His tag标签抗体 |
别 名 | HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine. |
Specific References (17) | bs-0287R has been referenced in 17 publications.
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产品类型 | 标签抗体 |
研究领域 | 肿瘤 细胞生物 免疫学 染色质和核信号 信号转导 合成与降解 结合蛋白 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Recombinant protein |
产品应用 | WB=1:1000-5000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | Synthetic peptide: HHHHHH conjugated to KLH |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
SWISS: N/A Gene ID: N/A 标签抗体 蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白 1) 6xHis与不同的Sepharose纯化填料结合(捆绑),可不受构象的影响,在天然或变形的条件下可进行进一步纯化。 |
产品图片 |
Sample:
Lane1: His-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Lane2: His-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Primary: Anti-His tag (bs-0287R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD
Sample:
Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 2ug Lane 2: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4ug Primary: Anti-His tag (bs-0287R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |