[IF=11.092] Juan Cen. et al. A Water-Soluble Quercetin Conjugate with Triple Targeting Exerts Neuron-Protective Effect on Cerebral Ischemia by Mitophagy Activation. ADV HEALTHC MATER. 2022 Sep;:2200817  IHC ;  Rat.  

PubMed:36071574

[IF=10.723] Jia-xin Hao. et al. A seminal perspective on the role of chondroitin sulfate in biomineralization. CARBOHYD POLYM. 2023 Jun;310:120738  IF ;  Rat.  

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[IF=10.652] Junjie Liu. et al. A specific “switch-on” type magnetic resonance nanoprobe with distance-dominate property for high-resolution imaging of tumors. Chem Eng J. 2021 Jan;404:126496  IHC ;  Mouse.  

PubMed:10.1016/j.cej.2020.126496

[IF=4.879] Petra Žádníková. et al. The Degradation of Hyaluronan in the Skin. Biomolecules. 2022 Feb;12(2):251  WB,IHC,IF ;  Human.  

PubMed:35204753

Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Hyaluronidase-1 Polyclonal Antibody, Unconjugated(bs-1235R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Hyaluronidase-1 Polyclonal Antibody, Unconjugated(bs-1235R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Blank control:Hela. Primary Antibody (green line): Rabbit Anti-Hyaluronidase-1 antibody (bs-1235R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 2μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.