[IF=4.249] Zoratto, F. et al.Intranasal oxytocin administration promotes emotional contagion and reduces aggression in a mouse model of callousness. (2018) Neuropharmacology.Sep 10;143:250-267.  IHC ;  mouse.  

PubMed:30213592

[IF=3.252] Ting-Wang Xiong. et al. Beneficial effects of Dendrobium nobile Lindl. Alkaloids (DNLA) on anxiety and depression induced by chronic unpredictable stress in rats. Brain Res. 2021 Nov;1771:147647  IHC ;  rat.  

PubMed:34481787

[IF=3.216] Daneng Wei. et al. The Volatile Oil of Zanthoxylum bungeanum Pericarp Improved the Hypothalamic-Pituitary-Adrenal Axis and Gut Microbiota to Attenuate Chronic Unpredictable Stress-Induced Anxiety Behavior in Rats. Drug Des Dev Ther. 2021 Feb;15:769-786  IHC ;  Rat.  

PubMed:33654382

[IF=1.69] Guo et al. Androgen receptor reverts dexamethasone?induced inhibition of prostate cancer cell proliferation and migration. (2018) Mol.Med.Rep. 17:5887-5893  WB ;  Human.  

PubMed:29436611

[IF=1.41] Pang et al. Expression of glucocorticoid receptor-α in the epidermis of patients with psoriasis vulgaris. (2015) Exp.Ther.Med. 10:419-422  IHC ;  Human.  

PubMed:26622331

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glucocorticoid receptor) Polyclonal Antibody, Unconjugated (bs-0252R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GCR) Polyclonal Antibody, Unconjugated (bs-0252R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glucocorticoid receptor) Polyclonal Antibody, Unconjugated (bs-0252R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Cell: H-4-II-E
Concentration:1:100
Host/Isotype:Rabbit/IgG
Flow cytometric analysis of primary antibody (Cat#: bs-0252R) on H-4-II-E(green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .

Blank control:A549.
Primary Antibody (green line): Rabbit Anti-Glucocorticoid receptor antibody (bs-0252R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.