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Mouse Anti-GAPDH(3E12)-Loading Control antibody (bsm-0978M)
说明书: 50ul  100ul  200ul

产品编号 bsm-0978M
英文名称 GAPDH(3E12)-Loading Control
中文名称 3-磷酸甘油醛脱氢酶单克隆抗体(内参抗体)
别    名 38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase;Glyceraldehyde 3 phosphate dehydrogenase liver;Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38 component; OCT1 coactivator in S phase, 38-KD component; wu:fb33a10.  
Specific References  (13)     |     bsm-0978M has been referenced in 13 publications.
[IF=3.03] Liu, Si-jun, et al. "Neuroprotective effect of β-asarone against Alzheimer’s disease: regulation of synaptic plasticity by increased expression of SYP and GluR1." Drug design, development and therapy 10 (2016): 1461.  WB ;  Rat.  
[IF=2.09] Di, Yu, et al. "CCN1/Cyr61-PI3K/AKT signaling promotes retinal neovascularization in oxygen-induced retinopathy." International journal of molecular medicine 36.6 (2015): 1507-1518.  WB ;  Human, Mouse.  
[IF=2.86] Pei, Haitao, et al. "The Effect of Minimally Invasive Hematoma Aspiration on the JNK Signal Transduction Pathway after Experimental Intracerebral Hemorrhage in Rats." International journal of molecular sciences 17.5 (2016): 710.  WB ;  Rat.  
[IF=3.33] Zhao, Hai-hua, et al. "Involvement of GSK3 and PP2A in ginsenoside Rb1's attenuation of aluminum-induced tau hyperphosphorylation." Behavioural Brain Research (2012).  WB ;  Mouse.  
[IF=2.80] Kang, Hongyan, et al. "A Potential Gravity-Sensing Role of Vascular Smooth Muscle Cell Glycocalyx in Altered Gravitational Stimulation." Astrobiology (2013).  WB ;  Rat.  
[IF=2.13] Wei, Ting, et al. "The role of N-acetylglucosaminyltransferases V in the malignancy of human hepatocellular carcinoma." Experimental and molecular pathology (2012).  WB ;  Human.  
[IF=2.38] Zhang, Xiao‐Qun, et al. "Regulation of pulmonary surfactant synthesis in fetal rat type II alveolar epithelial cells by microRNA‐26a." Pediatric Pulmonology (2014).  WB ;  Rat.  
[IF=4.44] Katoh, Hiroshi, et al. "Heat shock protein 90 ensures efficient mumps virus replication by assisting with viral polymerase complex formation." Journal of Virology (2017): JVI-02220.  WB ;  Monkey.  
[IF=5.62] Zhao, Tianyun, et al. "Ketamine administered to pregnant rats in the second trimester causes long-lasting behavioral disorders in offspring." Neurobiology of Disease (2014).  WB ;  Rat.  
[IF=0.65] Xu, Xiaochun, et al. "PPARγ, FAS, HSL mRNA and protein expression during Tan sheep fat-tail development." Electronic Journal of Biotechnology (2015).  WB ;  Sheep.  
[IF=0.65] Xu, Xiaochun, et al. "mRNA transcription and protein expression of PPARγ, FAS, and HSL in different parts of the carcass between fat-tailed and thin-tailed sheep." Electronic Journal of Biotechnology (2015).  WB ;  Sheep.  
[IF=3.08] Cui, Yanjun, and Xianhong Gu. "Proteomic changes of the porcine small intestine in response to chronic heat stress." Journal of Molecular Endocrinology (2015): JME-15.  WB ;  
[IF=1.27] Liu, Yong, et al. "Lentivirus-mediated TGF-β3, CTGF and TIMP1 gene transduction as a gene therapy for intervertebral disc degeneration in an in vivo rabbit model." Experimental and Therapeutic Medicine.  WB ;  Rabbit.  
规格价格 50ul/580元 购买    100ul/920元 购买    200ul/1600元 购买    大包装/询价
说 明 书 50ul  100ul  200ul
产品类型 内参抗体 
研究领域 肿瘤  免疫学  神经生物学  信号转导  通道蛋白  新陈代谢  Alzheimer's  
抗体来源 Mouse
克隆类型 Monoclonal
克 隆 号 3E12
交叉反应 Human, Mouse, Rat, Rabbit, 
产品应用 WB=1:500-5000 ELISA=1:1000-5000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 38kDa
细胞定位 细胞核 细胞浆 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 Full length GAPDH protein purified from rabbit muscle: 
亚    型 IgG
纯化方法 affinity purified by Protein G
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
Loading Control
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with EIF1AD, USP25, PRKCI and WARS.

Subcellular Location:
Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions.

Post-translational modifications:
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated (Probable).
Sulfhydration at Cys-152 increases catalytic activity.

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.


Gene ID:

Database links:

Entrez Gene: 374193 Chicken

Entrez Gene: 2597 Human

Entrez Gene: 100042025 Mouse

Entrez Gene: 14433 Mouse

Entrez Gene: 24383 Rat

Entrez Gene: 685186 Rat

Entrez Gene: 317743 Zebrafish

Omim: 138400 Human

SwissProt: P00356 Chicken

SwissProt: P04406 Human

SwissProt: P16858 Mouse

SwissProt: P04797 Rat

SwissProt: Q5XJ10 Zebrafish

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

GAPDH蛋白几乎在所有组织中都高水平表达,广泛用作Western blot蛋白质标准化的内参,是很好的内参抗体。
GAPDH 作为管家基因在同种细胞或者组织中的蛋白质表达量一般是恒定的。在实验中,可能存在总蛋白浓度测定不准确;或者蛋白质样品在电泳前上样时产生的样品间的操作误差;这些误差需要通过测定每个样品中实际转到膜上的GAPDH的含量来进行校正,所以一般的western实验都需要进行内参设置。具体校正的方法就是将每个样品测得的目的蛋白含量与本样品的GAPDH含量相除,得到每个样品目的蛋白的相对含量。然后才进行样品与样品之间的比较。
甘油醛-3-磷酸脱氢酶(Glyceraldehyde 3 phosphate dehydrogenase,GAPDH)是糖酵解(glycolysis)过程中的关键酶。除了在胞质中作为糖酵解的酶以外,有证据表明哺乳动物细胞中的GAPDH参与了多种胞内生化过程,包括膜融合(membrane fusion)、微管成束(microtubule bundling)、磷酸转移酶(phosphotransferase)激活、核内RNA出核、DNA复制与DNA修复。一些生理因素,诸如低氧(hypoxia)和尿糖(diabetes),可以增加GAPDH在特定细胞中的表达。GAPDH存在于几乎所有的组织中,以高水平持续表达。
Placenta (Mouse) Lysate at 40 ug
293T Cell Lysate at 40 ug
Primary: Anti-GAPDH(bsm-0978M)at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 38kD
Observed band size: 38kD
Sample: Bladder(Rat)Lysate at 40 ug
Primary: Anti-GAPDH(bsm-0978M)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 38kD
Observed band size: 38kD
Sample:Liver(Mouse)Lysate at 40 ug
Primary: Anti-GAPDH(bsm-0978M)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38kD
Observed band size: 38kD
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-GAPDH(3E12)-Loading Control (bsm-0978M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 38 kD
Paraformaldehyde-fixed, paraffin embedded (Rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH(3E12)-Loading Control) Polyclonal Antibody, Unconjugated (bsm-0978M) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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