产品编号 | bs-0017M |
英文名称 | Mouse Anti-GAL antibody |
中文名称 | 甘丙肽/神经节肽抗体 |
别 名 | GALA_HUMAN; Galanin peptides; Galanin; Galanin message-associated peptide (GMAP); GAL1; GALN; GLNN; galanin and GMAP prepropeptide; ETL8; GMAP; GAL-GMAP; |
研究领域 | 心血管 免疫学 神经生物学 糖尿病 内分泌病 |
抗体来源 | Mouse |
克隆类型 | Polyclonal |
交叉反应 | Rat (predicted: Human,Mouse,Pig,Sheep,Cow,Chicken,Dog,GuineaPig,Horse) |
产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 45364kDa |
细胞定位 | 分泌型蛋白 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human GAL: 33-62/123 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Galanin is small neuropeptide that functions as a cellular messenger within the central and peripheral nervous systems, modulating diverse physiologic functions (Mechenthaler, 2008 [PubMed 18500643]).[supplied by OMIM, Jan 2009] Function: Contracts smooth muscle of the gastrointestinal and genitourinary tract, regulates growth hormone release, modulates insulin release, and may be involved in the control of adrenal secretion. Subcellular Location: Secreted. Similarity: Belongs to the galanin family. SWISS: P22466 Gene ID: 51083 Database links: Entrez Gene: 51083 Human Entrez Gene: 14419 Mouse Omim: 137035 Human SwissProt: P22466 Human SwissProt: P47212 Mouse Unigene: 278959 Human Unigene: 4655 Mouse Unigene: 8929 Rat 神经节肽(Galanin)是由30个氨基酸残基组成的神经肽。最初认为只在相当小的背根神经节细胞群体及脊髓神经元中出现,后发现在消化道及胰腺中也广泛分布。与感觉的调整有关,对痛觉传递及脊髓兴奋起抑制作用,在很多禽类动物中有表达,是中枢神经系统及内分泌胰腺的神经调节肽。 |
产品图片 |
Tissue/cell: rat spinal cord; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Galanin Polyclonal Antibody, Unconjugated(bs-0017M) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0024) and DAB(C-0010) staining
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |