| 产品编号 | bs-1170R |
| 英文名称 | FOS B Rabbit pAb |
| 中文名称 | FosB抗体 |
| 别 名 | FBJ murine osteosarcoma viral oncogene homolog B; FOS B; FOS-B; FBJ osteosarcoma oncogene B; fosB; G0/G1 switch regulatory protein 3; FosB; G0/G1 switch regulatory protein 3; G0S3; GOS3; GOS 3; GOS B; GOSB; MGC42291; Oncogene FOS B; Oncogene FOSB; Protein fos B; Protein fosB; DKFZp686C0818; FOSB_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 免疫学 染色质和核信号 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat (predicted: Human,Mouse,Rabbit,Pig,Cow,Dog,GuineaPig,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 36 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FOS B: 191-290/338 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Cellular oncogenes, or proto-oncogenes, play pivotal roles in cellular communication pathways that regulate normal growth, development and differentiation. The cellular oncogene families fos and jun encode nuclear proteins that can function as transcription factors. The fos family of nuclear oncogenes encode cFos, Fos B, (fos-related antigen) Fra 1, and Fra 2. Also named as DKFZp686C0818; FBJ murine osteosarcoma viral oncogene homolog B; FosB; G0/G1 switch regulatory protein 3; G0S3; GOS3; GOSB; MGC42291; Oncogene FOS B; Protein fosB. Function: FosB interacts with Jun proteins enhancing their DNA binding activity. Subunit: Heterodimer. Subcellular Location: Nucleus. Similarity: Belongs to the bZIP family. Fos subfamily. Contains 1 bZIP (basic-leucine zipper) domain. SWISS: P53539 Gene ID: 2354 Database links: Entrez Gene: 2354 Human Omim: 164772 Human SwissProt: P53539 Human Unigene: 590958 Human 转录调节因子（Transcriptin Regulators） FOS B属于转录因子AP-1家族;FosB是一种转录因子，表达后能在脑内维持数周甚至几个月。长期药物滥用能引起伏核内FosB表达稳定地增加，表明FosB的表达可能是药物成瘾的分子转换机制，是当今药物成瘾研究中最受重视的转录因子。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FOS B) Polyclonal Antibody, Unconjugated (bs-1170R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
