| 产品编号 | bs-0788R |
| 英文名称 | intestinal FABP Rabbit pAb |
| 中文名称 | 肠型脂肪酸结合蛋白抗体 |
| 别 名 | FABP 2; FABP2; FABPI; Fatty acid binding protein 2 intestinal; Fatty acid binding protein intestinal; Fatty acid-binding protein; I-FABP; IFABP; Intestinal fatty acid binding protein 2; MGC133132; FABPI_HUMAN. |
| 研究领域 | 心血管 细胞生物 免疫学 生长因子和激素 糖尿病 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Pig,Chicken,Dog) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 15 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from IFABP: 45-132/132 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Intestinal FABP is an intestinal marker. It is expressed in the small intestine. Expression in the mucosal cells of the ileum extends from the midvillar region to the villus tips. It is thought to play a role in the intracellular transport of long chain fatty acids and their acyl CoA esters and may be involved in triglyceride rich lipoprotein synthesis. Function: FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor. Subcellular Location: Cytoplasm. Tissue Specificity: Expressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum. Similarity: Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. Database links: Entrez Gene: 2169 Human Entrez Gene: 14079 Mouse Omim: 134640 Human SwissProt: P12104 Human SwissProt: P55050 Mouse Unigene: 282265 Human Unigene: 28398 Mouse Unigene: 91358 Rat Fabp2脂肪酸结合蛋白-2,参与脂肪酸代谢、运输、消化，该蛋白的异常增高使人的新陈代谢功能异常，影响人体消耗摄入的糖分，从而导致引发糖尿病的发生。FABP2多态性可能与2型糖尿病合并冠心病的胰岛素抵抗相关联。 |
| 产品图片 |
Sample: Intestinal(Mouse) Lysate at 30ug;
Primary: Anti-intestinal FABP (bs-0788R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size: 15 kD
Observed band size: 17 kD
Sample: Raji Cell Lysate at 30ug;
Primary: Anti-intestinal FABP (bs-0788R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size: 15 kD
Observed band size: 17 kD
Tissue/cell: human colon carcinoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IFABP Polyclonal Antibody, Unconjugated(bs-0788R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
