| 产品编号 | bs-2428R |
| 英文名称 | Rabbit Anti-IL-9 antibody |
| 中文名称 | 白介素9抗体 |
| 别 名 | Interleukin-9; IL9; Homolog of mouse T cell and mast cell growth factor 40; HP40; IL 9; Mast cell growth factor; MCGF; Megakaryoblast growth factor; p40 cytokine; p40 T cell and mast cell growth factor; p40 T-cell and mast cell growth factor; T cell growth factor 3; T cell growth factor p40; T-cell growth factor p40; Interleukin 9; TCGF 3. |
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Specific References (2) | bs-2428R has been referenced in 2 publications.
[IF=3.7] Yao, Xiuhua, et al. "Neutralization of IL‐9 ameliorates symptoms of experimental autoimmune myasthenia gravis in rats by decreasing effector T cells and altering humoral responses." Immunology (2014). other ; Rat.
[IF=1.5] Lin, Yinyao, et al. "Expression of interleukin-9 and its upstream stimulating factors in rats with ischemic stroke." Neurological Sciences (2015): 1-8. IHC-P ; Rat.
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| 研究领域 | 肿瘤 细胞生物 免疫学 神经生物学 信号转导 细胞凋亡 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:200-800
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 16kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse IL-9: 21-120/144 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a cytokine that actsas a regulator of a variety of hematopoietic cells. This cytokinestimulates cell proliferation and prevents apoptosis. It functionsthrough the interleukin 9 receptor (IL9R), which activatesdifferent signal transducer and activator (STAT) proteins and thusconnects this cytokine to various biological processes. The geneencoding this cytokine has been identified as a candidate gene forasthma. Genetic studies on a mouse model of asthma demonstratedthat this cytokine is a determining factor in the pathogenesis ofbronchial hyperresponsiveness. [provided by RefSeq, Jul 2008]. Function: Supports IL-2 independent and IL-4 independent growth of helper T-cells. Subcellular Location: Secreted. Similarity: Belongs to the IL-7/IL-9 family. SWISS: P15247 Gene ID: 16198 Database links: Entrez Gene: 3578 Human Entrez Gene: 16198 Mouse Omim: 146931 Human SwissProt: P15248 Human SwissProt: P15247 Mouse Unigene: 960 Human Unigene: 3006 Mouse 白介素-9是由CD4和辅助性T细胞的Th2细胞分泌的细胞因子,具有多种生物学活性，在机体的免疫应答中具有多种免疫调节作用，IL-9参与肿瘤发生、寄生虫免疫、支气管哮喘、慢性阻塞性肺病和器官移植等多种疾病过程。 |
| 产品图片 |
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-IL-9 (bs-2428R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 16 kD
Observed band size: 16 kD
Paraformaldehyde-fixed, paraffin embedded (mouse colon tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-9) Polyclonal Antibody, Unconjugated (bs-2428R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IL-9 Polyclonal Antibody, Unconjugated(bs-2428R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse mammary tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-9) Polyclonal Antibody, Unconjugated (bs-2428R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-cy3) for 90 minutes, and DAPI for nuclei staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
