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Rabbit Anti-Egr1  antibody (bs-1076R)

~~~促销,代码KT240603~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-1076R
英文名称 Rabbit Anti-Egr1  antibody
中文名称 早期生长应答蛋白1抗体
别    名 Egr-1; Early growth response 1; Krox-24; Ngf1; Ngfi; NGFI-A; zif-268; Egr1; A530045N19Rik; egr; Egr-1; ETR103; Krox-1; Krox-24; Krox24; NGF1-A; NGFI-A; NGFIA; TIS8; Zenk; Zfp-6; AT 225; AT225; Early growth response protein 1; G0S 30; G0S30; KROX 24; Krox 24 protein; KROX24; TIS 8; TIS8; Transcription factor ETR 103; Transcription factor ETR103; Transcription factor Zif 268; Transcription factor Zif268; Zfp 6; ZIF268; Zinc finger protein 225; ZNF 225; ZNF225; EGR1_HUMAN.  
Specific References  (2)     |     bs-1076R has been referenced in 2 publications.
[IF=6.832] Hou, Yonghui. et al. Nonwoven-based gelatin/polycaprolactone membrane loaded with ERK inhibitor U0126 for treatment of tendon defects. Stem Cell Res Ther. 2022 Dec;13(1):1-11  IHC ;  Rat.  
[IF=2.209] Fan, Haobo. et al. Expression of early growth responsive gene-1 in the visual cortex of monocular form deprivation amblyopic kittens. Bmc Ophthalmol. 2021 Dec;21(1):1-8  IHC ;  Kittens.  
研究领域 细胞生物  免疫学  信号转导  生长因子和激素  细胞周期蛋白  转录调节因子  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse,Rat
产品应用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=2ug/test,ICC=1:100,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 60kDa
细胞定位 细胞核 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Egr-1: 401-500/453 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. [provided by RefSeq].

Function:
Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.

Subcellular Location:
Nucleus.

Similarity:
Belongs to the EGR C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.

SWISS:
P18146

Gene ID:
1958

Database links:

Entrez Gene: 1958 Human

Entrez Gene: 13653 Mouse

Omim: 128990 Human

SwissProt: P18146 Human

SwissProt: P08046 Mouse

Unigene: 326035 Human

Unigene: 708393 Human

Unigene: 181959 Mouse



Egr-1为即刻早期基因家族中的一个成员,是一种含3个锌指结构的转录因子。有研究表明EGR-1在细胞生长分化中发挥重要作用,如在细胞周期G1-S期的转换过程中Egr-1被诱导表达。
产品图片
Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-Egr1 (bs-1076R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD
Sample: Liver (Rat) Lysate at 40 ug Primary: Anti- Egr1 (bs-1076R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Egr1) Polyclonal Antibody, Unconjugated (bs-1076R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Egr1 Polyclonal Antibody, Unconjugated(bs-1076R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat small intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Egr1 Polyclonal Antibody, Unconjugated(bs-1076R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Egr1) polyclonal Antibody, Unconjugated (bs-1076R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Egr1 Antibody(bs-1076R) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.Cells stained with primary antibody (green), and isotype control (orange).
Blank control:K562. Primary Antibody (green line): Rabbit Anti-Egr1 antibody (bs-1076R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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