产品编号 | bs-0596R |
英文名称 | Rabbit Anti-Dopamine beta Hydroxylase antibody |
中文名称 | 多巴胺β羟化酶抗体 |
别 名 | DBH; DBM; Dopamine beta hydroxylase; Dopamine-Beta-Hydroxylase; Dopamine beta monooxygenase; DOPO_RAT; DOPO_HUMAN. |
研究领域 | 肿瘤 神经生物学 合成与降解 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human (predicted: Mouse,Rat) |
产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 70kDa |
细胞定位 | 细胞浆 细胞膜 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human DBH: 471-550/621 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Dopamine-beta-Hydroxylase (DBH), Dopamine can be transformed into Noradrenalin. Conversion of dopamine to noradrenaline. [SUBUNIT] Homotetramer composed of two non-covalently bound disulfide-linked dimers. [SUBCELLULAR LOCATION] Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen. Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single-pass type II membrane protein (Potential). Belongs to the copper type II ascorbate-dependent monooxygenase family. Function: Conversion of dopamine to noradrenaline. Subunit: Homotetramer composed of two non-covalently bound disulfide-linked dimers (By similarity). Subcellular Location: Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen (By similarity). Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen (By similarity). Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein (By similarity). Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single-pass type II membrane protein (By similarity). Tissue Specificity: Chromaffin granules of the adrenal medulla and synaptic vesicles of the sympathetic nervous system. Similarity: Belongs to the copper type II ascorbate-dependent monooxygenase family. Contains 1 DOMON domain. SWISS: P09172 Gene ID: 1621 Database links: Entrez Gene: 1621 Human Entrez Gene: 13166 Mouse Omim: 223360 Human Omim: 609312 Human SwissProt: P09172 Human SwissProt: Q64237 Mouse Unigene: 223858 Human Unigene: 591890 Human Unigene: 167781 Mouse Unigene: 87166 Rat Dopamine-β-Hydroxylase(DBH)—多巴胺β羟化酶。可以将多巴胺转化成去甲肾上腺素。是肾上腺素能神经元的标志。 |
产品图片 |
Sample:
Jurkat (Human) Cell Lysate at 40 ug
Primary: Anti-Dopamine beta Hydroxylase (bs-0596R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 70 kD
Observed band size: 70 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DOPO) Polyclonal Antibody, Unconjugated (bs-0596R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control(black line):SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-Dopamine beta Hydroxylase antibody (bs-0596R)
Dilution:1ug/Test;
Secondary Antibody(white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control(orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |