[IF=12.02] Wakiyama H. et al. Treg-Dominant Tumor Microenvironment Is Responsible for Hyperprogressive Disease after PD-1 Blockade Therapy.. CANCER IMMUNOL RES. 2022 Nov;10(11):1386-1397  IHC ;  Mouse.  

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[IF=3.118] Qianqian Wu. et al. Protection of melatonin against long‐term radon exposure‐caused lung injury. Environ Toxicol. 2021 Apr;36(4):472-483  

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[IF=2.923] Junyuan Ma. et al. Effects of melatonin on development and hormone secretion of sheep theca cells in vitro. THERIOGENOLOGY. 2023 Mar;198:172  IF ;  Sheep.  

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Tissue/cell: mouse embryos tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cytokeratin 1 Polyclonal Antibody, Unconjugated(bs-1244R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cytokeratin 1 Polyclonal Antibody, Unconjugated(bs-1244R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Blank control (blue line): Hela cells(blue).
Primary Antibody (green line): Rabbit Anti-Cytokeratin 1/FITC Conjugated antibody (bs-1244R-PE)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-PE.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃ . The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.

Blank control (blue line): Rabbit spleen cells (blue).
Primary Antibody (green line): Rabbit Anti-Cytokeratin 1/PE Conjugated antibody (bs-1244R-PE)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-PE .
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃ . The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.

Blank control (blue line): MCF7 cells(blue).
Primary Antibody (green line): Rabbit Anti-Cytokeratin 1/FITC Conjugated antibody (bs-1244R-FITC)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-FITC.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃ . The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.