| 产品编号 | bs-1009R |
| 英文名称 | CXCR1 |
| 中文名称 | 细胞表面趋化因子受体1抗体 |
| 别 名 | C C; C C CKR 1; CCCKR1; CD 181; CD128; CD181; CDw128a; Chemokine (C X C motif) receptor 1; Chemokine (C X C) receptor 1; Chemokine receptor 1; CKR 1; CMKAR1; CXCR 1; High affinity interleukin 8 receptor A; IL 8 receptor type 1; IL 8R A; IL-8RA; IL8 receptor; IL8 receptor type 1; IL8R1; IL8RA; IL8RBA; Interleukin 8 receptor alpha; Interleukin 8 receptor type 1. Interleukin 8 receptor type A; CXCR1_HUMAN; C-C; CD181; CKR-1; IL8R1; CDw128a; C-C-CKR-1. |
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Specific References (6) | bs-1009R has been referenced in 6 publications.
[IF=8.786] Guangxu Xiao. et al. CXCR1 and its downstream NF-κB inflammation signaling pathway as a key target of Guanxinning injection for myocardial ischemia/reperfusion injury.. FRONT IMMUNOL. 2022 Oct;13:1007341-1007341 WB, IHC, IF ; Mouse.
[IF=3.37] Chengfei Xu. et al. CXCR1 participates in bone cancer pain induced by Walker 256 breast cancer cells in female rats. MOL PAIN. ;(): WB, IF ; Rat.
[IF=2.57] Schneberger, D., et al. "CXCR1/CXCR2 antagonist CXCL8 (3-74) K11R/G31P blocks lung inflammation in swine barn dust-instilled mice." Pulmonary Pharmacology & Therapeutics (2015). IHC-P ; Mouse.
[IF=2.24] Tang, Cui, et al. "High expression of GPER1, EGFR and CXCR1 is associated with lymph node metastasis in papillary thyroid carcinoma." International Journal of Clinical and Experimental Pathology 7.6 (2014): 3213-3223. IHC-P ; Human.
[IF=2.24] Tang et al. High expression of GPER1, EGFR and CXCR1 is associated with lymph node metastasis in papillary thyroid carcinoma. (2014) Int.J.Clin.Exp.Patho. 7:3213-23 IHC ; Human.
[IF=1.89] Zhao, Le, et al. "Role of GPER1, EGFR and CXCR1 in differentiating between malignant follicular thyroid carcinoma and benign follicular thyroid adenoma."International Journal of Clinical and Experimental Pathology 8.9 (2015): 11236-11247. IHC-P ; Human.
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| 研究领域 | 肿瘤 免疫学 细胞膜受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Dog,Cow,Rabbit) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 40kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CXCR1: 186-280/350 <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. Knockout studies in mice suggested that this protein inhibits embryonic oligodendrocyte precursor migration in developing spinal cord. This gene, IL8RB, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. [provided by RefSeq, Jul 2008] Function: Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL-8 with a high affinity and to MGSA (GRO) with a low affinity. Subcellular Location: Cell membrane. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P25024 Gene ID: 3577 Database links: Entrez Gene: 3577 Human Entrez Gene: 227288 Mouse Omim: 146929 Human SwissProt: P25024 Human SwissProt: Q810W6 Mouse Unigene: 194778 Human Unigene: 337035 Mouse Unigene: 138115 Rat CXCR1主要表达于浸润的炎性细胞和肠上皮细胞表面. |
| 产品图片 |
Sample:
Lane 1: Mouse Lymph node tissue lysates Lane 2: Rat Lymph node cell lysates Primary: Anti-CXCR1 (bs-1009R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kDa Observed band size: 45 kDa 
Sample:
Lane 1: Mouse Lung tissue lysates Lane 2: Human Jurkat cell lysates Lane 3: Human Raji cell lysates Primary: Anti-CXCR1 (bs-1009R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kDa Observed band size: 45 kDa 
Tissue/cell: rat colitis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CXCR1/IL-8RA Polyclonal Antibody, Unconjugated(bs-1009R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
