| 产品编号 | bs-0582R |
| 英文名称 | Cyclooxygenase 1 |
| 中文名称 | 前列腺素氧化环化酶1抗体 |
| 别 名 | Cyclooxygenase-1; COX-1; COX 1; COX1; Cyclooxygenase1; Prostaglandin-endoperoxide synthase-1; prostaglandin-endoperoxide synthase 1; PTGS-1; Prostaglandin G/H synthase 1 precursor; prostaglandin G/H synthase 1 isoform 1 precursor; Prostaglandin H2 synthase 1; PGH synthase 1; PGHS-1; PHS 1; PGH1_HUMAN; PTGS1; Prostaglandin G/H synthase 1 precursor. |
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Specific References (4) | bs-0582R has been referenced in 4 publications.
[IF=2.86] Li, Wei, et al. "Combined effects of cyclooxygenase-1 and cyclooxygenase-2 selective inhibitors on ovarian carcinoma in vivo." International journal of molecular sciences 12.1 (2011): 668-681 WB ; Mouse.
[IF=2.38] Hu, Ruiqi, et al. "The expression of prostaglandin-E2 and its receptor in the oviduct of Chinese brown frog (Rana dybowskii)." Prostaglandins & Other Lipid Mediators (2016). IHC-P ; Others.
[IF=2.253] Chen Z et al. Seasonal expressions of prostaglandin E synthases and receptors in the prostate of the wild ground squirrel (Spermophilus dauricus). Prostaglandins and Other Lipid Mediators 148 (2020) 106412. IHC-P ; squirrel.
[IF=2.253] Wang Y et al. Seasonal expressions of COX-1, COX-2 and EP4 in the uteri of the wild Daurian ground squirrels (Spermophilus dauricus). Prostaglandins Other Lipid Mediat. 2019 Aug;143:106343. IHC&WB ; squirrel.
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| 研究领域 | 肿瘤 心血管 免疫学 信号转导 合成与降解 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Dog,Pig,Cow,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 63kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cyclooxygenase 1: 151-250/599 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This is one of two genes encoding similar enzymes that catalyze the conversion of arachinodate to prostaglandin. The encoded protein regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. The protein may promote cell proliferation during tumor progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012]. Function: May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells. Subunit: Homodimer. Subcellular Location: Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Similarity: Belongs to the prostaglandin G/H synthase family. Contains 1 EGF-like domain. SWISS: Q63921 Gene ID: 5742 Database links: Entrez Gene: 5742 Human Entrez Gene: 19224 Mouse Omim: 176805 Human SwissProt: P23219 Human SwissProt: P22437 Mouse Unigene: 201978 Human Unigene: 275434 Mouse Unigene: 44404 Rat 合成与降解（Synthesis and Degradation） COX1 前列腺素氧化环化酶-是前列腺素合成的关键酶之一，为诱导性酶，他参与细胞对生长因子、肿瘤促进因子和细胞因子的反应，这些因子均能诱导COX1表达，它也参与前列腺素的合成。 |
| 产品图片 |
Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug A431(Human) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug Huvec(Human) Cell Lysate at 30 ug Primary: Anti-Cyclooxygenase 1 (bs-0582R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kD Observed band size: 63 kD 
Tissue/cell: human laryngo carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-COX-1 Polyclonal Antibody, Unconjugated(bs-0582R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
