| 产品编号 | bs-0552R |
| 英文名称 | COL5A1 Rabbit pAb |
| 中文名称 | Ⅴ型胶原抗体 |
| 别 名 | EDSC; EDSCL1; FMDMF; CO5A1_HUMAN; COL5A1; |
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Specific References (5) | bs-0552R has been referenced in 5 publications.
[IF=17.521] Yingni Xu. et al. Biomimetic Convex Implant for Corneal Regeneration Through 3D Printing. Advanced Science. 2023 Feb;:2205878 IF ; Rabbit.
[IF=4.315] Tong Xu. et al. A signature of circadian rhythm genes in driving anaplastic thyroid carcinoma malignant progression. CELL SIGNAL. 2022 Jul;95:110332 WB ; Human.
[IF=3.662] Xiaoting Lu. et al. Porous hydrogel constructs based on methacrylated gelatin/polyethylene oxide for corneal stromal regeneration. MATER TODAY COMMUN. 2022 Aug;32:104071 IF ; Rabbit.
[IF=2.586] R S N Barreto. et al. THE EXTRACELLULAR MATRIX PROTEIN PATTERN IN THE CANINE NEOPLASTIC MAMMARY GLAND. TISSUE CELL. 2023 Feb;:102050 IHC ; Dog.
[IF=0] Kanthilal, Manisha. Characterization of Molecular and Mechanical Phenotypes of Freshly Isolated Lipoaspirate Cells. Diss. Brown University, 2013. FCM ; Human.
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| 研究领域 | 肿瘤 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Chicken,Dog) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 153 kDa |
| 细胞定位 | 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human COL5A1: 1401-1500/1838 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013] Function: Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Subunit: Trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Interacts with CSPG4. Subcellular Location: Secreted, extracellular space, extracellular matrix. Post-translational modifications: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Sulfated on 40% of tyrosines. DISEASE: Ehlers-Danlos syndrome 1 (EDS1) [MIM:130000]: A connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome. Note=The disease is caused by mutations affecting the gene represented in this entry. Ehlers-Danlos syndrome 2 (EDS2) [MIM:130010]: Mild form of classic Ehlers-Danlos syndrome. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the fibrillar collagen family. Contains 1 fibrillar collagen NC1 domain. Contains 1 laminin G-like domain. SWISS: P20908 Gene ID: 1289 Database links: Entrez Gene: 1289 Human SwissProt: P20908 Human |
| 产品图片 |
Sample:
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Skin tissue lysates
Lane 3: Mouse Uterus tissue lysates
Lane 4: Rat Uterus tissue lysates
Lane 5: Human A549 cell lysates
Primary: Anti-COL5A1 (bs-0552R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 153 kDa
Observed band size: 200 kDa
Tissue/cell: rat skin tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Collagen V Polyclonal Antibody, Unconjugated(bs-0552R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human colon carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GM-CSF Polyclonal Antibody, Unconjugated(bs-0552R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
