[IF=5.9] Zhao, Jing, et al. "Chronic obstructive sleep apnea causes atrial remodeling in canines: mechanisms and implications." Basic Research in Cardiology 109.5 (2014): 1-13.  WB ;  Dog.  

PubMed:25015734

[IF=3.501] Zeng H et al. Transcriptomic analysis of α-synuclein knockdown after T3 spinal cord injury in rats. BMC Genomics. 2019 Nov 14;20(1):851.  IHF-F ;  Rat.  

PubMed:31726970

[IF=2.624] Liyang Tong. et al. Topical bendazol inhibits experimental myopia progression and decreases the ocular accumulation of HIF‐1α protein in young rabbits. Ophthal Physl Opt. 2020 Sep;40(5):567-576  WB ;  Rabbit.  

PubMed:32839973

[IF=1.642] Tong L et al. Effects of topical pilocarpine on ocular growth and refractive development in rabbits. Eur J Ophthalmol. 2020 Jun 11;1120672120934962.  IHC-P&WB ;  Rabbit.  

PubMed:32524847

Sample:
Lane 1: Mouse Spinal cord tissue lysates
Lane 2: Mouse Heart tissue lysates
Lane 3: Rat Spinal cord tissue lysates
Lane 4: Rat Heart tissue lysates
Primary: Anti- ChRM2 (bs-0441R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 47 kDa

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ChRM2 Polyclonal Antibody, Unconjugated(bs-0441R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ChRM2 Polyclonal Antibody, Unconjugated(bs-0441R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Blank control: Hela(blue).
Primary Antibody:Rabbit Anti- ChRM2 antibody(bs-0441R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Antibody (bs-0441R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-0441R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.