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CHGA Rabbit pAb (bs-0539R)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-0539R
英文名称 CHGA Rabbit pAb
中文名称 嗜铬粒素A抗体
别    名 Chromogranin-A; Chromogranin A; beta Granin; CGA; CHG A; Chromogranin A parathyroid secretory protein 1; Chromogranin A precursor; ChromograninA; Pancreastatin; Parastatin; Parathyroid secretory protein 1; Pituitary secretory protein I; SP I; SP1; SPI; Vasostatin; ER-37; CMGA_HUMAN.  
Specific References  (9)     |     bs-0539R has been referenced in 9 publications.
[IF=8.39] Liu, Yanchun, et al. "HB-EGF Embedded in PGA/PLLA Scaffolds via Subcritical CO 2 Augments the Production of Tissue Engineered Intestine."Biomaterials (2016).  IF(IHC-P) ;  Rat.  
[IF=8.101] Shuang Xie. et al. Indispensable role of melatonin, a scavenger of reactive oxygen species (ROS), in the protective effect of Akkermansia muciniphila in cadmium-induced intestinal mucosal damage. FREE RADICAL BIO MED. 2022 Nov;193:447  IF ;  Mouse.  
[IF=5.7] Wang, Yuli, et al. "In vitro generation of colonic epithelium from primary cells guided by microstructures." Lab on a Chip (2014).  Mouse.  
[IF=5.7] Wang et al. In vitro generation of colonic epithelium from primary cells guided by microstructures. (2014) Lab.Chi. 14:1622-31  ICC ;  Mouse.  
[IF=5.7] Wang, Yuli, et al. "Capture and 3D culture of colonic crypts and colonoids in a microarray platform." Lab on a Chip 13.23 (2013): 4625-4634.  IF(IHC-F) ;  Mouse.  
[IF=5.295] Ko-Chao Lee. et al. Use of iTRAQ-based quantitative proteomic identification of CHGA and UCHL1 correlated with lymph node metastasis in colorectal carcinoma. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2023 May;:  IHC,WB ;  Human.  
[IF=5.011] Geng-xiu Zan. et al. Heat stress disrupts intestinal stem cell migration and differentiation along the crypt–villus axis through FAK signaling. BBA-MOL CELL RES. 2023 Mar;1870:119431  IHC ;  Mouse.  
[IF=4.25] Liu, Yanchun, et al. "Enriched Intestinal Stem Cell Seeding Improves the Architecture of Tissue Engineered Intestine." Tissue Engineering (2014).  IHC-P ;  Rat.  
[IF=3.26] Liu, Xin-Qi, et al. "Regulation of neuroendocrine cells and neuron factors in the ovary by zinc oxide nanoparticles." Toxicology Letters (2016).  IHC-P ;  Chicken.  
研究领域 肿瘤  
抗体来源 Rabbit
克隆类型 Polyclonal
克 隆 号
交叉反应 Human,Rat (predicted: Mouse)
产品应用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 48 kDa
检测分子量
细胞定位 细胞浆 细胞膜 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CHGA: 421-470/457 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown. [provided by RefSeq, Jul 2008].

Function:
Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.

Subunit:
Interacts with SCG3.

Subcellular Location:
Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle membrane. Secreted. Note=Associated with the secretory granule membrane through direct interaction to SCG3 that in turn binds to cholesterol-enriched lipid rafts in intragranular conditions.

Post-translational modifications:
Sulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans.

Similarity:
Belongs to the chromogranin/secretogranin proteinfamily.

SWISS:
P10645

Gene ID:
1113

Database links:

Entrez Gene: 1113 Human

Entrez Gene: 12652 Mouse

Omim: 118910 Human

SwissProt: P10645 Human

SwissProt: P26339 Mouse

Unigene: 150793 Human



嗜铬粒素A蛋白存在与很多组织中:胃肠道内分泌细胞和类癌、甲状旁腺、垂体前叶、肾上腺髓质、神经节、副神经节、胰岛、甲状腺C细胞等组织和其来源的肿瘤都表达该成分.嗜铬素A(CgA)属于可溶性的酸性蛋白,主要存在于神经内分泌细胞的分泌颗粒中,也存在于含有分泌颗粒的内分泌细胞和神经内分泌细胞来源的肿瘤细胞中。检测循环中的免疫反应CgA对神经内分泌瘤具有诊断意义。另外,CgA的免疫组织化学检测对鉴定来源于神经内分泌的肿瘤起到辅助作用。
产品图片
Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Chromogranin A) Polyclonal Antibody, Unconjugated (bs-0539R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Chromogranin A) Polyclonal Antibody, Unconjugated (bs-0539R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Chromogranin A) Polyclonal Antibody, Unconjugated (bs-0539R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Chromogranin A) polyclonal Antibody, Unconjugated (bs-0539R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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