| 产品编号 | bs-0298R |
| 英文名称 | IGBP1 Rabbit pAb |
| 中文名称 | 免疫球蛋白结合蛋白-1抗体 |
| 别 名 | alpha 4; B cell signal transduction molecule alpha 4; B-cell signal transduction molecule alpha 4; bA351K23.1; CD79a binding protein 1; CD79a-binding protein 1; IBP 1; IBP1; IGBP 1; Igbp1; IGBP1_HUMAN; Immunoglobulin(CD79A) binding protein 1; Immunoglobulin binding protein 1; Immunoglobulin-binding protein 1; Protein alpha 4; Protein alpha-4; Protein phosphatase 2A regulatory subunit alpha 4; Renal carcinoma antigen NY REN 16; Renal carcinoma antigen NY-REN-16. |
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Specific References (2) | bs-0298R has been referenced in 2 publications.
[IF=4.21] Luo, Guangying, et al. "Paternal bisphenol a diet changes prefrontal cortex proteome and provokes behavioral dysfunction in male offspring." Chemosphere (2017). WB ; Mouse.
[IF=2.515] Li T et al. Digital gene expression analyses of mammary glands from meat ewes naturally infected with clinical mastitis. R. Soc. open sci. 2019,6: 181604. IHC-P ; Sheep.
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| 研究领域 | 肿瘤 细胞生物 免疫学 神经生物学 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Rat (predicted: Mouse,Pig,Cow,GuineaPig) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 44 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Immunoglobulin binding protein 1: 151-250/339 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The proliferation and differentiation of B cells is dependent upon a B-cell antigen receptor (BCR) complex. Binding of antigens to specific B-cell receptors results in a tyrosine phosphorylation reaction through the BCR complex and leads to multiple signal transduction pathways. [provided by RefSeq, Jul 2008]. Function: Associated to surface IgM-receptor; may be involved in signal transduction. Involved in regulation of the catalytic activity of PP2A, PP4 and PP6 phosphatases catalytic subunits by protecting them from degradative polyubiquitination until they associate with regulatory subunits. Subunit: Associates with PP2A-alpha and PP2A-beta catalytic subunits, and with PP4 and PP6. Interacts with MID1 and MID2. Interacts with Ubiquitin. Subcellular Location: Cytoplasm (Potential). Tissue Specificity: Ubiquitously expressed with highest levels in heart, skeletal muscle and pancreas. Post-translational modifications: Phosphorylated. Monoubiquitination by MID1 triggers calpain-mediated cleavage and switches IGBP1 activity from protective to destructive. DISEASE: Mental retardation, X-linked, syndromic, 28 (MRXS28) [MIM:300472]: A mental retardation syndrome characterized by agenesis of the corpus callosum, coloboma of the iris and optic nerve, severe retrognathia, and intellectual deficit. Mental retardation is defined by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the IGBP1/TAP42 family. Contains 1 UIM (ubiquitin-interacting motif) repeat. SWISS: P78318 Gene ID: 3476 Database links: Entrez Gene: 3476 Human Entrez Gene: 18518 Mouse Omim: 300139 Human SwissProt: P78318 Human SwissProt: Q61249 Mouse Unigene: 496267 Human Unigene: 7454 Mouse Unigene: 7079 Rat CD79a表达于从前B细胞到浆细胞的整个B细胞发育阶段。 CD79a与CD79b构成B细胞受体复合体，在介导IgM从细胞内转到细胞膜上起重要作用。CD79a是B细胞常用标记物。CD79复合物是异二聚体分子，由CD79a/mb-1和CD79b/B29 组成。在B细胞表面非共价地与膜结合的免疫球蛋白相连，构成B细胞抗原受体。在大多数前B细胞急性淋巴细胞白血病、B细胞系、B细胞淋巴瘤和和某些骨髓瘤中发现有CD79a的存在。主要用于B淋巴细胞的判断和B细胞淋巴瘤的诊断。 |
| 产品图片 |
Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IGBP1 Polyclonal Antibody, Unconjugated(bs-0298R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control（black line）:HepG2.
Primary Antibody (green line): Rabbit Anti-IGBP1 antibody (bs-0298R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
