| 产品编号 | bs-0736R |
| 英文名称 | CD56 Rabbit pAb |
| 中文名称 | 神经细胞粘附分子1抗体 |
| 别 名 | CD56 antigen; Cell Adhesion Molecule Neural 1; MSK39; N CAM 120; N CAM 140; N CAM 180; NCAM 1; NCAM 120; NCAM 140; NCAM1; Neural cell adhesion molecule 1 120 kDa isoform; Neural cell adhesion molecule 1 140 kDa isoform; Neural cell adhesion molecule 1; NCAM1_HUMAN. |
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Specific References (3) | bs-0736R has been referenced in 3 publications.
[IF=5.68] Shi, Liang, et al. "Targeting Androgen Receptor (AR)→ IL12A Signal Enhances Efficacy of Sorafenib Plus NK Cells-Immunotherapy to Better Suppress HCC Progression." Molecular Cancer Therapeutics (2016): molcanther-0706. IHC-P ; Human.
[IF=3.886] Huiqian Zeng. et al. Increased uterine NLRP3 inflammasome and leucocyte infiltration in a rat model of preeclampsia. 2021 Aug 10 IHC ; Rat.
[IF=3.26] Liu, Xin-Qi, et al. "Regulation of neuroendocrine cells and neuron factors in the ovary by zinc oxide nanoparticles." Toxicology Letters (2016). IHC-P ; Chicken.
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| 研究领域 | 细胞生物 神经生物学 干细胞 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Rat (predicted: Mouse,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 92 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD56/NCAM1: 701-800/858 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011] Function: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Subcellular Location: Isoform 1, 2,: Cell membrane; Single-pass type I membrane protein. Isoform 3, 4: Cell membrane; Lipid-anchor, GPI-anchor. Isoform 5, 6: Secreted. Similarity: Contains 2 fibronectin type-III domains. Contains 5 Ig-like C2-type (immunoglobulin-like) domains. SWISS: P13591 Gene ID: 4684 Database links: Entrez Gene: 4684 Human Entrez Gene: 17967 Mouse Omim: 116930 Human SwissProt: P13591 Human SwissProt: P13595 Mouse Unigene: 503878 Human Unigene: 711235 Human Unigene: 733031 Human Unigene: 439182 Mouse Unigene: 4974 Mouse Unigene: 11283 Rat 细胞粘附蛋白（Call Adhesion Protein） 神经元标志物 NCAM-1为神经细胞粘附分子，主要分布于神经组织，神经—肌肉接头，神经—内分泌腺和某些内分泌腺以及大多数神经外胚层来源的细胞、组织和肿瘤中。 NCAM 1主要用于视网膜母细胞瘤、髓母细胞瘤、星形细胞瘤、神经母细胞瘤等肿瘤方面的研究。CD56（神经细胞黏附分子，NCAM）表达于大部分神经外胚层来源的细胞系、组织和肿瘤如视网膜母细胞瘤、成神经管细胞瘤、星形细胞瘤以及成神经细胞瘤。它也表达于一些中胚层来源的肿瘤如横纹肌肉瘤。NK细胞以及NK细胞淋巴瘤为阳性。CD56被认为是NK细胞肿瘤的重要标志物。该抗体识别分子量为180，145和125kDa亚型。 |
| 产品图片 |
Protein: Hela(human)cell lysates at 40ug;
Primary: rabbit Anti-CD56 (bs-0736R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size:92 kD
Observed band size:125 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat se
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
