客服热线:400-901-9800          客服QQ:4009019800          技术答疑     技术支持     质量反馈     联系我们
首页 > 产品中心 > 一抗 > 产品信息
Rabbit Anti-CD31 antibody (bs-0468R)
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购传真:010-58129612
技术支持:support@bioss.com.cn
说明书: 50ul  100ul  200ul
20ul/480.00元
50ul/880.00元
100ul/1580.00元
200ul/2480.00元
大包装/询价

产品编号 bs-0468R
英文名称 CD31
中文名称 血小板内皮细胞黏附分子-1抗体
别    名 platelet endothelial cell adhesion molecule precursor-1; PECAM-1; PECAM1; Adhesion molecule; CD31 antigen; CD31 EndoCAM; Endocam; FLJ34100; FLJ58394; GPIIA; Pecam 1; PECA1_HUMAN; PECAM 1 CD31 EndoCAM; PECA1; Pecam1; Platelet endothelial cell adhesion molecule; Platelet/endothelial cell adhesion molecule 1; Adhesion molecule; Platelet/endothelial cell adhesion molecule.  
Specific References  (11)     |     bs-0468R has been referenced in 11 publications.
[IF=2.56] Yuan, Qiao-ying, et al. "A targeted high-efficiency angiogenesis strategy as therapy for myocardial infarction." Life Sciences (2012).  IHC-P ;  
[IF=1.37] Zhang, Jun, et al. "Cardiac stem cells differentiate into sinus node-like cells." The Tohoku Journal of Experimental Medicine 222.2 (2010): 113-120.  Dog.  
[IF=5.29] Guo, Yansu, et al. "A Single Injection of Recombinant Adeno-Associated Virus into the Lumbar Cistern Delivers Transgene Expression Throughout the Whole Spinal Cord." Molecular Neurobiology (2015): 1-14.  IHC-F ;  Mouse.  
[IF=3.87] Ma, Yan, et al. "Intraocular expression of serum amyloid a and interleukin-6 in proliferative diabetic retinopathy." American journal of ophthalmology 152.4 (2011): 678-685.  IHC-F ;  Mouse.  
[IF=5.47] Guo, Yansu, et al. "A single injection of recombinant adeno-associated virus into the lumbar cistern delivers transgene expression throughout the whole spinal cord." Molecular Neurobiology (2015): 1-14.  IHC ;  Mouse.  
[IF=2.57] Vuletic, Ivan, et al. "Validation of Bevacizumab Therapy Effect on Colon Cancer Subtypes by Using Whole Body Imaging in Mice." Molecular Imaging and Biology: 1-10.  IHC-F ;  Mouse.  
[IF=2.45] Yuan, Qiao-Ying, et al. "A visible, targeted high-efficiency gene delivery and transfection strategy." BMC biotechnology 11.1 (2011): 56.  IHC-P ;  Dog.  
[IF=3.04] Korkmaz, H. Ibrahim, et al. "Neutrophil Extracellular Traps Coincide with a Pro‐coagulant Status of Microcirculatory Endothelium in Burn Wounds." Wound Repair and Regeneration (2017).  IHC-P ;  Pig.  
[IF=3.22] Long, Connor, et al. "Isolation and characterization of canine placenta‐derived mesenchymal stromal cells for the treatment of neurological disorders in dogs." Cytometry Part A (2017).  FCM ;  Dog.  
[IF=8.65] Chen, Xiang, et al. "A small interfering CD147-targeting RNA inhibited the proliferation, invasiveness, and metastatic activity of malignant melanoma." Cancer research 66.23 (2006): 11323-11330.  Mouse.  
[IF=3.73] Zeng, Wei-qun, et al. "A New Method to Isolate and Culture Rat Kupffer Cells." PLOS ONE 8.8 (2013): e70832.  Rat.  
规格价格 50ul/880元 购买    100ul/1580元 购买    200ul/2480元 购买    大包装/询价
说 明 书 50ul  100ul  200ul
研究领域 肿瘤  心血管  细胞生物  免疫学  信号转导  干细胞  细胞粘附分子  细胞表面分子  糖蛋白  淋巴细胞  t-淋巴细胞  骨髓细胞  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Horse, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1μg/Test 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 78kDa
细胞定位 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD31:601-680/738 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
Platelet endothelial cell adhesion molecule (PECAM-1) also known as cluster of differentiation 31 (CD31) is a protein that in humans is encoded by the PECAM1 gene found on chromosome 17. PECAM-1 plays a key role in removing aged neutrophils from the body. PECAM-1 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte transmigration, angiogenesis, and integrin activation.

Function:
Induces susceptibility to atherosclerosis. Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).

Subunit:
Interacts with PTPN11; Tyr-713 is critical for PTPN11 recruitment. Forms a complex with BDKRB2 and GNAQ. Interacts with BDKRB2 and GNAQ.

Subcellular Location:
Isoform Long: Membrane; Single-pass type I membrane protein. Cell junction. Note=Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
Isoform Delta15: Cell junction. Note=Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.

Tissue Specificity:
Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).

Post-translational modifications:
Phosphorylated on Ser and Tyr residues after cellular activation. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.

Similarity:
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

SWISS:
P16284

Gene ID:
5175

Database links:

Entrez Gene: 5175 Human

Entrez Gene: 18613 Mouse

Omim: 173445 Human

SwissProt: P16284 Human

SwissProt: Q08481 Mouse

Unigene: 376675 Human

Unigene: 514412 Human

Unigene: 343951 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

细胞粘附蛋白(Call Adhesion Protein)
血小板内皮细胞黏附分子-1 在血小板、内皮细胞、单核细胞、嗜中性细胞及某些T细胞亚群上表达的质膜糖蛋白。
属于免疫球蛋白超基因家族成员,在细胞外结构域中有6个C2亚类免疫球蛋白样保守性同原单位。在炎症应答中起作用。
产品图片
Protein: U937(huamn)Cell lysates;
Primary: Anti-CD31(bs-0468R) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 5000; ECL excitated the fluorescence; Predicted band size :78kD Observed band size : 85kD
Blank control: HUVEC cells(blue).
Primary Antibody:Rabbit Anti-CD31 antibody(bs-0468R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) .Primary antibody (bs-0468R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Cell:R.spleen
Concentration:1:100
Host/Isotype:Rabbit/IgG
Flow cytometric analysis of Rabbit IgG isotype control (Cat#: bs-0468R) on R.spleen(green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .
版权所有 2016-2020 www.bioss.com.cn 北京博奥森生物技术有限公司 京ICP备05066980号 京公网安备110107000727号
通过国际质量管理体系ISO9001:2008 GB/T19001-2008认证    编号:00115Q211807R1M/1100