| 产品编号 | bs-1168R |
| 英文名称 | CCR4 |
| 中文名称 | 细胞表面趋化因子受体4抗体 |
| 别 名 | CC chemokine receptor 4; chemokine (C-C motif) receptor 4; CC-CKR-4; CCR-4; CD194; CHEMOKINE CC4 RECEPTOR; CHEMR1; ChemR13; CKR4; CMKBR4; HGCN:14099; K5-5; LESTR; MGC151418; MGC88293; nocturnin, Sdf1r; chemokine C C motif receptor 4; ChemR13; CCR4_HUMAN; C-C chemokine receptor type 4; K5-5; CD 194; CD-194. |
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Specific References (3) | bs-1168R has been referenced in 3 publications.
[IF=5.793] Nicolas Pelisch. et al. CCL3 contributes to secondary damage after spinal cord injury. J Neuroinflamm. 2020 Dec;17(1):1-16
[IF=4.223] Han, Dongxu. et al. Subacute cadmium exposure modulates Th1 polarization to trigger ER stress-induced porcine hepatocyte apoptosis via regulation of miR-369-TNFα axis. 2021 Oct 14 IF ; Pig.
[IF=4.2] Ge, L. P., et al. "Integration of nondegradable polystyrene and degradable gelatin in a core–sheath nanofibrous patch for pelvic reconstruction." International Journal of Nanomedicine 2015:10 3193–3201 IHC-P ; Rat.
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| 研究领域 | 细胞生物 细胞膜受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 40kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CCR-4: 201-300/360 <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene belongs to the G-protein-coupled receptor family . It is a receptor for the CC chemokine - MIP-1, RANTES, TARC and MCP-1. Chemokines are a group of small polypeptide, structurally related molecules that regulate cell trafficking of various types of leukocytes. The chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. [provided by RefSeq, Jul 2008] Function: High affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Predominantly expressed in the thymus, in peripheral blood leukocytes, including T-cells, mostly CD4+ cells, and basophils, and in platelets; at lower levels, in the spleen and in monocytes. Detected also in macrophages, IL-2-activated natural killer cells and skin-homing memory T-cells, mostly the ones expressing the cutaneous lymphocyte antigen (CLA). Expressed in brain microvascular and coronary artery endothelial cells. Post-translational modifications: In natural killer cells, CCL22 binding induces phosphorylation on yet undefined Ser/Thr residues, most probably by beta-adrenergic receptor kinases 1 and 2. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P51679 Gene ID: 1233 Database links: Entrez Gene: 1233 Human Entrez Gene: 12773 Mouse GenBank: NP_005499.1 Human Omim: 604836 Human SwissProt: P51679 Human SwissProt: P51680 Mouse Unigene: 184926 Human Unigene: 1337 Mouse 趋化因子是当今细胞因子研究领域的热点之一,它参与多种免疫及炎症反应,在感染、肿瘤的生长与转移、组织修复及创伤愈合等病理生理过程中发挥重要作用. C-C型趋化因子受体4(CCR4)在Th2细胞表面有大量表达.CCR4在肿瘤的生长与转移方面起到一定的作用。 |
| 产品图片 |
Sample:
Lane 1: Bone (Mouse) Lysate at 40 ug Lane 2: Bone (Rat) Lysate at 40 ug Primary: Anti-CCR4 (bs-20788R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD 
Tissue/cell: human hepatitides tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CCR4 Polyclonal Antibody, Unconjugated(bs-1168R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining 
Tissue/cell: rat exfoliated cells; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CCR4 Polyclonal Antibody, Unconjugated(bs-1168R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
