| 产品编号 | bs-2242R |
| 英文名称 | Rabbit Anti-BMP7 antibody |
| 中文名称 | 骨形态发生蛋白7抗体 |
| 别 名 | bone morphogenetic protein 7 precursor; osteogenic protein 1; BMP-7; OP-1; BMP 7; Bone morphogenic protein 7; Eptotermin alfa; OP 1; OP1; BMP7_HUMAN. |
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Specific References (4) | bs-2242R has been referenced in 4 publications.
[IF=5.959] Wang Y et al. SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway. Cell Death Dis. 2019 Nov 7;10(11):852. WB ; Mouse.
[IF=5.31] Shuxin Liu. et al. Overexpression of bone morphogenetic protein 7 reduces oligodendrocytes loss and promotes functional recovery after spinal cord injury. 2021 Aug 13 WB ; Rat.
[IF=3.54] Mai et al. Dyssynchronous pacing triggers endothelial-mesenchymal transition through heterogeneity of mechanical stretch in a canine model. (2014) Circ.. 79:201-9 WB ; Canine.
[IF=3.309] Gulistan Sanem Saribas. et al. Ellagic acid increases implantation rates with its antifibrotic effect in the rat model of intrauterine adhesion. PATHOL RES PRACT. 2023 Jun;246:154499 IHC ; Rat.
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| 研究领域 | 肿瘤 细胞生物 免疫学 细胞凋亡 生长因子和激素 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat,Rabbit (predicted: Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BMP7: 293-350/431 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development and possible bone inductive activity. [provided by RefSeq]. Function: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. Subunit: Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with TWSG1. Subcellular Location: Secreted. Tissue Specificity: Expressed in the kidney and bladder. Lower levels seen in the brain. Post-translational modifications: Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (PubMed:17977014). Similarity: Belongs to the TGF-beta family. SWISS: P18075 Gene ID: 655 Database links: Entrez Gene: 655 Human Entrez Gene: 12162 Mouse Omim: 112267 Human SwissProt: P18075 Human SwissProt: P23359 Mouse Unigene: 473163 Human Unigene: 595 Mouse Unigene: 18030 Rat 骨形态发生蛋白7（BMP-7）是转化生长因子β超家族成员,参与器官发生,细胞增殖、分化及凋亡等. |
| 产品图片 |
Sample:
Bone (Mouse) Lysate at 40 ug
Primary: Anti-BMP7 (bs-2242R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15.7/47 kD
Observed band size: 47,50kD
Sample:
Lane 1: Kidney (Mouse) Lysate at 40 ug
Lane 2: Placenta (Mouse) Lysate at 40 ug
Lane 3: Cerebrum (Mouse) Lysate at 40 ug
Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 5: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-BMP7 (bs-2242R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
Sample:
293T Cell (Human) Lysate at 30 ug
Primary: Anti-BMP7 (bs-2242R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15.7/47kD
Observed band size: 49/40 kD
Tissue/cell: rabbit alveolar bone; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-BMP7 Polyclonal Antibody, Unconjugated(bs-2242R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
