| 产品编号 | bs-0600R |
| 英文名称 | ADRA1A |
| 中文名称 | alpha 1肾上腺素能受体A抗体 |
| 别 名 | ADA1A_HUMAN; Adrenergic alpha 1A receptor; Adrenergic alpha 1C receptor; Adrenergic alpha 1D receptor; alpha 1 Adrenergic Receptor; Alpha 1A adrenergic receptor; Alpha-1A adrenergic receptor; Alpha-1A adrenoreceptor; Alpha-1C adrenergic receptor; Alpha-adrenergic receptor 1c; ADRA1A; ADRA1C; Alpha 1A adrenoceptor; alpha-1A adrenergic receptor isoform 1; adrenergic, alpha-1A-, receptor variant 1; adrenergic, alpha-1A-, receptor variant 3; adrenergic, alpha-1A-, receptor variant 5; adrenergic, alpha-1A-, receptor variant 8; G protein coupled receptor; alpha-1A adrenoceptor; ADRA1L1; ALPHA1AAR. |
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Specific References (4) | bs-0600R has been referenced in 4 publications.
[IF=7.84] Chen, Li-You, et al. "Early-life sleep deprivation persistently depresses melatonin production and bio-energetics of the pineal gland: potential implications for the development of metabolic deficiency." Brain Structure and Function (2014): 1-14. WB ; Rat.
[IF=4.37] Wang, Wenjuan, et al. "Effects of Estradiol Valerate and Remifemin on Norepinephrine Signaling in the Brain of Ovariectomized Rats."Neuroendocrinology 101.2 (2015): 120-132. IHC-F ; Rat.
[IF=2.59] Sun, Tao, et al. "Antihypertensive effect of formononetin through regulating the expressions of eNOS, 5-HT< sub> 2A/1B receptors and α< sub> 1-adrenoceptors in spontaneously hypertensive rat arteries." European Journal of Pharmacology (2013). Rat.
[IF=2.532] Jianchao Ren. et al. Ejaculatory Duct Obstruction Affects Seminal Vesicle Contractile Efficacy and Smooth Muscle Ultrastructure in a Rat Model. ANDROLOGIA. 2023;2023:5022466 WB ; Rat.
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| 研究领域 | 细胞生物 神经生物学 细胞膜受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,GuineaPig) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 51kDa |
| 细胞定位 | 细胞核 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Alpha-1A adrenergic receptor: 201-300/466 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties. [provided by RefSeq, Jul 2008]. Function: This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes. Subunit: Homo- and heterooligomer. Heterooligomerizes with ADRA1B homooligomers in cardiac myocytes. Subcellular Location: Nucleus membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note=Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes. Tissue Specificity: Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethral and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart. Post-translational modifications: C-terminal Ser or Thr residues may be phosphorylated. Similarity: Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1A sub-subfamily. SWISS: P35348 Gene ID: 148 Database links: Entrez Gene: 148 Human Entrez Gene: 11549 Mouse Omim: 104221 Human SwissProt: P35348 Human SwissProt: P97718 Mouse Unigene: 52931 Human Unigene: 709175 Human Unigene: 57064 Mouse Unigene: 9991 Rat ADRA1肾上腺素能α1受体位于突触后，在血管平滑肌上，兴奋时可使血管收缩; alpha 1-adrenergic receptor有兴奋效应也有抑制效应。肾上腺素能受体又可分为α和β两种。alpha 受体与儿茶酚胺结合后，主要是兴奋平滑肌，如血管收缩、子宫收缩和瞳孔开张肌收缩等；但也有抑制作用，如使小肠平滑肌舒张。β受体又可分为β1和β2两个亚型. |
| 产品图片 |
Sample:
Lane 1: Mouse Heart tissue lysates Lane 2: Rat Heart tissue lysates Primary: Anti-ADRA1A (bs-0600R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kDa Observed band size: 47 kDa 
Sample:
Heart (Mouse) Lysate at 40 ug Primary: Anti-ADRA1A (bs-0600R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 62 kD 
Sample:
U937 Cell (Human) Lysate at 30 ug Raji Cell (Human) Lysate at 30 ug Primary: Anti-ADRA1A (bs-0600R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 55 kD 
Sample:
kidney (Mouse) Lysate at 40 ug Primary: Anti-ADRA1A (bs-0600R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 62 kD 
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ADRA1/ADRA1B/alpha 1 Adrenergic Receptor Polyclonal Antibody, Unconjugated (bs-0600R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining 
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ADRA1/ADRA1B/alpha 1 Adrenergic Receptor Polyclonal Antibody, Unconjugated (bs-0600R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
