| 产品编号 | bs-0039R |
| 英文名称 | ADNP Rabbit pAb |
| 中文名称 | 活性依赖的神经保护肽抗体 |
| 别 名 | Activity dependent neuroprotective protein; Activity dependent neuroprotector; NAP; KIAA0784 |
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Specific References (1) | bs-0039R has been referenced in 1 publications.
[IF=2.59] Ma, Xian-Cang, et al. "Intranasal Delivery of Recombinant NT4-NAP/AAV Exerts Potential Antidepressant Effect." Neurochemical Research: 1-6. Human.
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| 研究领域 | 细胞生物 神经生物学 细胞凋亡 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse,Rat (predicted: Human,Cow,Chicken,Dog) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 114 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ADNP: 331-400/1102 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The activity dependent neuroprotective protein (ADNP) gene is frequently amplified in many neoplasias, including breast, bladder, ovarian, pancreatic, and colon cancers. ADNP mRNA is abundantly expressed in distinct normal tissues, and high expression levels were encountered in malignant cells. ADNP is implicated in maintaining cell survival, perhaps through modulation of p53. The encoded protein contains one homeobox and nine zinc finger domains, suggesting that it functions as a transcription factor.
Function: Potential transcription factor. May mediate some of the neuroprotective peptide VIP-associated effects involving normal growth and cancer proliferation. Subcellular Location: Nucleus (Potential). Tissue Specificity: Widely expressed. Strong expression in heart, skeletal muscle, kidney and placenta. In brain, expression is stronger in the cerebellum and cortex regions. No expression detected in the colon. Strong increase of expression in colon and breast cancer tissues. Similarity: Contains 9 C2H2-type zinc fingers. Contains 1 homeobox DNA-binding domain. SWISS: Q9H2P0 Gene ID: 23394 Database links: Entrez Gene: 23394 Human Omim: 611386 Human SwissProt: Q9H2P0 Human Unigene: 570355 Human 是一个潜在的转录调节因子，参与正常细胞与肿瘤细胞的增殖调控。 广泛分布于大脑、小脑等组织，在肺、肾、小肠以及睾丸等组织也有微弱的表达。对体外培养的glia缺失神经元细胞有保护作用. |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADNP) Polyclonal Antibody, Unconjugated (bs-0039R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADNP) Polyclonal Antibody, Unconjugated (bs-0039R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
