| 产品编号 | bs-0433R |
| 英文名称 | ADAMTS7 Rabbit pAb |
| 中文名称 | 整合素样金属蛋白酶与凝血酶1型-7抗体 |
| 别 名 | A disintegrin and metalloproteinase with thrombospondin motifs 7; ADAM metallopeptidase with thrombospondin type 1 motif 7; A disintegrin and metalloprotease with thrombospondin motifs 7 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 7; A disintegrin like and metalloprotease(reprolysin type) with thrombospondin type 1 motif 7; A disintegrin like and metalloprotease with thrombospondin type 1 motif 7; ADAM metallopeptidase with thrombospondin type 1 motif 7; ADAM metallopeptidase with thrombospondin type 1 motif 7 preproprotein; ADAM TS 7; ADAM TS7; ADAMTS 7; ADAMTS7; COMPase; DKFZp434H204; ATS7_HUMAN. |
| 研究领域 | 细胞生物 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Rat (predicted: Mouse,Cow) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 158 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ADAMTS7: 1601-1686/1686 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Metalloprotease that may play a role in the degradation of COMP. Binds 1 zinc ion per subunit. pH dependence: Optimum pH is between 7.5 and 9.5. [Subcellular location] Secreted, extracellular space, extracellular matrix (By similarity). Note=Also found associated with the external cell surface[Tissue specificity] Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Detected in meniscus, bone, tendon, cartilage, synovium, fat and ligaments. Up-regulated in articular cartilage and synovium from arthritis patients. Function: Metalloprotease that may play a role in the degradation of COMP. Subunit: Interacts with COMP. Subcellular Location: Secreted, extracellular space, extracellular matrix. Note=Also found associated with the external cell surface. Tissue Specificity: Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Detected in meniscus, bone, tendon, cartilage, synovium, fat and ligaments. Post-translational modifications: N-glycosylated. O-glycosylated proteoglycan. Contains chondroitin sulfate (By similarity). May be cleaved by a furin endopeptidase (By similarity). The precursor is sequentially processed. Similarity: Contains 1 disintegrin domain. Contains 1 peptidase M12B domain. Contains 1 PLAC domain. Contains 8 TSP type-1 domains. SWISS: Q9UKP4 Gene ID: 11173 Database links: Entrez Gene: 11173 Human Omim: 605009 Human SwissProt: Q6P7J9 Human SwissProt: Q9UKP4 Human Unigene: 16441 Human 整合素样金属蛋白酶与凝血酶1型-7用于动脉粥样硬化导致早期血管基质修复作用的研究 |
| 产品图片 |
Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADAMTS7 Polyclonal Antibody, Unconjugated(bs-0433R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADAMTS7 Polyclonal Antibody, Unconjugated(bs-0433R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADAMTS7 Polyclonal Antibody, Unconjugated(bs-0433R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
