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Rabbit Anti-ACE  antibody (bs-0439R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-0439R
英文名称 ACE
中文名称 血管紧张素转换酶ACE1抗体
别    名 Angiotensin Converting Enzyme 1; ACE; ACE-T; Angiotensin-converting enzyme isoform 1precursor; Dipeptidyl carboxy peptidase 1; Kininase II; ACE-1;testis-specific isoform precursor. ACE 1; ACE T; ACE1; Angiotensin converting enzyme somatic isoform; Angiotensin converting enzyme testis specific isoform; Angiotensin I converting enzyme; Angiotensin I converting enzyme 1; Angiotensin I converting enzyme peptidyl dipeptidase A 1; Carboxycathepsin; CD 143; CD143; CD143 antigen; DCP 1; DCP; DCP1; Dipeptidyl carboxypeptidase 1; MVCD3; Peptidase P; Peptidyl dipeptidase A; Testicular ECA; ACE_HUMAN.  
Specific References  (14)     |     bs-0439R has been referenced in 14 publications.
[IF=4.996] Cong Changsheng. et al. Renin-angiotensin system inhibitors mitigate radiation pneumonitis by activating ACE2-angiotensin-(1–7) axis via NF-κB/MAPK pathway. SCI REP-UK. 2023 May;13(1):1-11  WB ;  Mouse.  
[IF=4.571] Shuwei Wang. et al. Formaldehyde causes an increase in blood pressure by activating ACE/AT1R axis. TOXICOLOGY. 2023 Mar;486:153442  IHC ;  Mouse.  
[IF=4.358] Deng T et al. Di-(2-ethylhexyl) phthalate induced an increase in blood pressure via activation of ACE and inhibition of the bradykinin-NO pathway. Environ Pollut. 2019 Apr;247:927-934.  IHC ;  Mouse.  
[IF=4.225] Gao Mengyu. et al. The Role of Nrf2 in the PM-Induced Vascular Injury Under Real Ambient Particulate Matter Exposure in C57/B6 Mice. Front Pharmacol. 2021 Feb;12:206  IHC ;  Mouse.  
[IF=4.014] Peng Wang. et al. ASSOCIATION ANALYSIS AND EXPRESSION LEVEL OF ACE POLYMORPHISMS WITH EGG-LAYING TRAIT IN TAIHANG CHICKEN. POULTRY SCIENCE. 2022 Sep;:102163  WB ;  Chicken.  
[IF=3.974] Xie X et al. Comparing the effects of diethylhexyl phthalate and dibutyl phthalate exposure on hypertension in mice.Ecotoxicol Environ Saf. 2019 Jun 15;174:75-82.  IHC ;  Mouse.  
[IF=3.448] Chen ZZ et al. Tanshinone IIA contributes to the pathogenesis of endometriosis via renin angiotensin system by regulating the dorsal root ganglion axon sprouting. Life Sci. 2019 Nov 20;240:117085.  IHC-P&WB ;  Rat.  
[IF=3.373] Zhang et al. Simultaneous targeting of ATM and Mcl-1 increases cisplatin sensitivity of cisplatin-resistant non-small cell lung cancer. (2017) Cancer.Biol.Ther. 18:606-615  WB ;  Human.  
[IF=3.263] Ademola Adetokunbo Oyagbemi et al. Antihypertensive power of Naringenin is mediated via attenuation of mineralocorticoid receptor (MCR)/ angiotensin converting enzyme (ACE)/ kidney injury molecule (Kim-1) signaling pathway. Eur J Pharmacol. 2020 Aug 5;880:173142.  IHC ;  Rat.  
[IF=3.166] Deng T et al. Exposure to diisononyl phthalate induced an increase in blood pressure through activation of the ACE/ AT1R axis and inhibition of NO production. Toxicol Lett. 2019 Jul;309:42-50.  IHC ;  Mouse.  
[IF=1.984] Liu H et al. Ginsenoside Rg3 Attenuates Angiotensin II-Mediated Renal Injury in Rats and Mice by Upregulating Angiotensin-Converting Enzyme 2 in the Renal Tissue. Evid Based Complement Alternat Med. 2019 Nov 29;2019:6741057.  IHC-P ;  Rat.  
[IF=1.92] Liu, Chen, et al. "Pulmonary artery denervation improves pulmonary arterial hypertension induced right ventricular dysfunction by modulating the local renin-angiotensin-aldosterone system." BMC Cardiovascular Disorders 16.1 (2016): 192.  WB ;  Dog.  
[IF=1.789] Xiaohua Liang . et al. Vitamin A deficiency indicating as low expression of LRAT may be a novel biomarker of primary hypertension. Clin Exp Hypertens. 2021;43(2):151-163  WB ;  Human.  
[IF=1.26] Jiang et al. Ginsenoside Rg3 induces ginsenoside Rb1-comparable cardioprotective effects independent of reducing blood pressure in spontaneously hypertensive rats. (2017) Exp.Ther.Med. 14:4977-4985  IHC-P ;  Rat.  
研究领域 肿瘤  心血管  细胞生物  免疫学  干细胞  细胞表面分子  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Mouse/Rat,Rat,Mouse,Human (predicted: Sheep,Cow,Pig,Dog)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 147kDa
细胞定位 细胞膜 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ACE1: 801-900/1306 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes an enzyme involved in blood pressure regulation and electrolyte balance. It catalyzes the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This angiotensin converting enzyme (ACE) also inactivates the vasodilator protein, bradykinin. Accordingly, the encoded enzyme increases blood pressure and is a drug target of ACE inhibitors, which are often prescribed to reduce blood pressure. This enzyme additionally plays a role in fertility through its ability to cleave and release GPI-anchored membrane proteins in spermatozoa. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme. This polymorphism, as well as mutations in this gene, have been implicated in a wide variety of diseases including cardiovascular pathophysiologies, psoriasis, renal disease, stroke, and Alzheimer's disease. Regulation of the homologous ACE2 gene may be involved in progression of disease caused by several human coronaviruses, including SARS-CoV and SARS-CoV-2. Alternative splicing results in multiple transcript variants encoding both somatic (sACE) and male-specific testicular (tACE) isoforms. [provided by RefSeq, Sep 2020]

Function:
Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.

Subcellular Location:
Angiotensin-converting enzyme, soluble form: Secreted.
Cell membrane; Single-pass type I membrane protein.

Tissue Specificity:
Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.

Post-translational modifications:
Phosphorylated by CK2 on Ser-1299; which allows membrane retention.

DISEASE:
Genetic variations in ACE may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
Defects in ACE are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
Genetic variations in ACE are associated with susceptibility to microvascular complications of diabetes type 3 (MVCD3) [MIM:612624]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.

Similarity:
Belongs to the peptidase M2 family.

SWISS:
P12821

Gene ID:
1636

Database links:

Entrez Gene: 1636 Human

Entrez Gene: 11421 Mouse

Omim: 106180 Human

SwissProt: P12821 Human

SwissProt: P09470 Mouse

Unigene: 298469 Human

Unigene: 754 Mouse



合成与降解(Synthesis and Degradation) ACE的主要功能是转化血管紧张素Ⅰ为血管紧张素Ⅱ,后者有升高血压的作用。
大多数结节病活动期ACE活性升高.
产品图片
Sample: Lane 1: Mouse Lung tissue lysates Lane 2: Mouse Testis tissue lysates Lane 3: Rat Lung tissue lysates Lane 4: Rat Testis tissue lysates Primary: Anti-ACE (bs-0439R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 147 kDa Observed band size: 110,180 kDa
Paraformaldehyde-fixed, paraffin embedded (human kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ACE) Polyclonal Antibody, Unconjugated (bs-0439R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-ACE1 Polyclonal Antibody, Unconjugated(bs-0439R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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