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Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Recombinant Rabbit mAb (bsm-62927R)  
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25ul/800.00元
50ul/1400.00元
100ul/2500.00元
大包装/询价

产品编号 bsm-62927R
英文名称 Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Recombinant Rabbit mAb
中文名称 磷酸化p21激活激酶1/2/3重组兔单抗
别    名 ADRB2; alpha-PAK; CDC42/RAC effector kinase PAK-A; EC 2.7.11.1; P65-PAK; P68-PAK; PAK1(phospho S144); PAK2(phospho S141); PAK3(phospho S139).  
抗体来源 Rabbit
克隆类型 Recombinant
克 隆 号 9G6
交叉反应 Human,Mouse,Rat
产品应用 WB=1:500-1:5000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:100-1:200,Flow-Cyt=1:20-1:100,ICC/IF=1:50-1:200,IP=1:20-1:50
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 58-62 kDa
检测分子量 65
性    状 Liquid
免 疫 原 A synthesized peptide derived from human PAK1 around the phosphorylation site of S144: YM-pS-FT 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
保存条件 Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation.

SWISS:
Q13153

Gene ID:
5058

PAK1-3蛋白具有广泛的生物学功能,也是一个保守的丝氨酸/苏氨酸蛋白激酶,参与许多重要的细胞活动。包括细胞骨架的动力学调节,细胞移动,生存和凋亡,细胞周期,基因转录调节,细胞生长信号转导和转化等。
产品图片
MCF-7 (H) cells were treated with or without EGF (1µg/ml) for 10 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) monoclonal antibody, unconjugated (bsm-62927R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154)  Monoclonal Antibody, Unconjugated(bsm-62927R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154)  Monoclonal Antibody, Unconjugated(bsm-62927R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154)  Monoclonal Antibody, Unconjugated(bsm-62927R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154)  Monoclonal Antibody, Unconjugated(bsm-62927R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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