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Phospho-MEK1 (Thr292) Recombinant Rabbit mAb (bsm-62900R)  
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25ul/800.00元
50ul/1400.00元
100ul/2500.00元
大包装/询价

产品编号 bsm-62900R
英文名称 Phospho-MEK1 (Thr292) Recombinant Rabbit mAb
中文名称 磷酸化MEK1重组兔单抗
别    名 Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1; MAP2K1; MEK-1; PRKMK1.  
抗体来源 Rabbit
克隆类型 Recombinant
克 隆 号
交叉反应 Human
产品应用 WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 43 kDa
检测分子量 45
性    状 Liquid
免 疫 原 A synthesized peptide derived from human MEK1 around the phosphorylation site of T292: PR-pT-PG 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
保存条件 Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules.

SWISS:
Q02750

Gene ID:
5604

产品图片
Jurkat (H) cells were treated with or without calyculin A (100 nM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-MEK1 (Thr292) monoclonal antibody, unconjugated (bsm-62900R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-MEK1 (Thr292) Monoclonal Antibody, Unconjugated(bsm-62900R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-MEK1 (Thr292) Monoclonal Antibody, Unconjugated(bsm-62900R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-MEK1 (Thr292) Monoclonal Antibody, Unconjugated(bsm-62900R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsils; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-MEK1 (Thr292) Monoclonal Antibody, Unconjugated(bsm-62900R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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