| 产品编号 | bsm-61475R |
| 英文名称 | ATG10 Recombinant Rabbit mAb |
| 中文名称 | 自噬相关蛋白10重组兔单抗 |
| 别 名 | APG10; APG10L; pp12616; 5330424L23Rik; 5430428K15Rik; Agp10; Apg10p; Atg10l; mAPG10; ATG10_HUMAN; ATG10; Autophagy-related protein 10 (APG10-like); 2.3.2.-; ATG10_MOUSE; Autophagy-related protein 10 (APG10-like | mAPG10); |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 11C4 |
| 交叉反应 | Human |
| 产品应用 | WB=1:1000-2000,Flow-Cyt=1:50-100,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 25 kDa |
| 检测分子量 | 25 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human ATG10: 170-220 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. SWISS: Q9H0Y0 Gene ID: 83734 |
| 产品图片 |
Western blot analysis of HeLa cell lysate. Using ATG10 (bsm-61475R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The K562 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-ATG10 antibody (bsm-61475R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
